Peer Review History

Original SubmissionMay 20, 2020
Decision Letter - Guy Smagghe, Editor

PONE-D-20-15116

Increased and sex-selective avian predation of Desert Locusts Schistocerca gregaria treated with Metarhizium acridum (Green Muscle®)

PLOS ONE

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Guy Smagghe, PhD

Academic Editor

PLOS ONE

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Reviewers' comments:

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Comments to the Author

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Reviewer #1: Yes

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2. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

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Reviewer #1: Yes

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Reviewer #1: Yes

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5. Review Comments to the Author

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Reviewer #1: The topic of the study is very significant considering the recent devastating effects of the desert locusts in the African continent.

The study was well structured, experiments setup as per standards. Authors also analyzed their data sufficiently.

Revisions:

The sentence in line 126 to 129 seems too long, maybe they could shorten.

In results section: some of the results were described after the table, it seemed confusing. It would be better if all results are described before table citation.

Besides the above two comments, I find the manuscript sound with significant findings that can contribute towards sustainable control of locust in Africa and globe.

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Reviewer #1: Yes: Amulen Deborah Ruth

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Revision 1

We are very grateful to the useful comments which will make the paper better understandable.

A. Editors remarks:

1. PlosOne's style requirements. We have verified the style and we believe it complies with the requirements.

2. Following the previous correspondence with the academic editor, we have removed the commercial name from the title but kept the scientific name of the fungus as this is a general species name and not linked to a specific isolate which has been commercialized. The title is now: "Increased and sex-selective avian predation of Desert Locusts Schistocerca gregaria treated with Metarhizium acridum".

3. The figure has been completely redrawn and the detailed map is no longer based on any existing cartography but it has been prepared by using our own field records and GPS coordinates.

B. Reviewers responses:

We thank the reviewer for her kind words as far as the importance of the paper is in relation to the ongoing devastating Desert Locust outbreak in Eastern Africa. We are grateful to her suggestions to improve the quality of the paper. Our replies are as follows:

1. no modifications necessary

2. no modifications necessary

3. no modifications necessary

4. "The sentence in line 126 to 129 seems too long, maybe they could shorten".

This has been revised as follows: [new lines 124 to128]

" Our hypotheses were:

(1) avian predation complements the impact of the fungal insecticide to control the locusts, and

(2) birds prefer the larger females of the desert locust over the males given that, in general, birds preferentially take larger species of grasshoppers or locusts or, within a species, the larger sex [29-31]".

In results section: "some of the results were described after the table, it seemed confusing. It would be better if all results are described before table citation".

We agree with the comments and all results are now placed before the figure.

C. In addition to the above mentioned modifications we have made a few minor changes :

In the introduction: The phrase "Although GM is no longer......marketed as NOVACRID (http://en.elephant......novacrid/) has been replaced by:

"A second strain of M.acridum (EVCH 077), marketed as NOVACRID, was registered in November 2019 by the Comité Sahélien des Pesticides for use in the Sahel". [new lines 92 and 93]

The caption of Fig 1 has been replaced by:

"Figure 1. Map of study site and location of transects A (SW-NE) and B (SE-NW). The greenness of the Schouwia vegetation is the situation by mid-November. The lines C1-C4 indicate the position of the spray papers".

In the acknowledgements we have added:

"We kindly acknowledge the comments of Dr Amulen Deborah Ruth on an earlier version of the manuscript and the preparation of the figures by Mr Dick Visser, University of Groningen, The Netherlands".

We trust that we have properly addressed all issues raised either by the editor or the reviewer

Decision Letter - Guy Smagghe, Editor

Increased and sex-selective avian predation of Desert Locusts Schistocerca gregaria treated with Metarhizium acridum

PONE-D-20-15116R1

Dear Dr. Mullié,

We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements.

Within one week, you’ll receive an e-mail detailing the required amendments. When these have been addressed, you’ll receive a formal acceptance letter and your manuscript will be scheduled for publication.

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Kind regards,

Guy Smagghe, PhD

Academic Editor

PLOS ONE

Additional Editor Comments (optional):

Reviewers' comments:

Formally Accepted
Acceptance Letter - Guy Smagghe, Editor

PONE-D-20-15116R1

Increased and sex-selective avian predation of Desert Locusts Schistocerca gregaria treated with Metarhizium acridum

Dear Dr. Mullié:

I'm pleased to inform you that your manuscript has been deemed suitable for publication in PLOS ONE. Congratulations! Your manuscript is now with our production department.

If your institution or institutions have a press office, please let them know about your upcoming paper now to help maximize its impact. If they'll be preparing press materials, please inform our press team within the next 48 hours. Your manuscript will remain under strict press embargo until 2 pm Eastern Time on the date of publication. For more information please contact onepress@plos.org.

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Thank you for submitting your work to PLOS ONE and supporting open access.

Kind regards,

PLOS ONE Editorial Office Staff

on behalf of

Prof. Guy Smagghe

Academic Editor

PLOS ONE

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