PONE-D-20-26579

Genetic analysis reveals an east-west divide within North American Vitis species that mirrors their resistance to Pierce's disease.

PLOS ONE

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PLOS ONE

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Comments to the Author

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Reviewer #1: Yes

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2. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

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Reviewer #1: Yes

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Reviewer #1: Yes

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5. Review Comments to the Author

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Reviewer #1: This study used an extensive sampling of Vitis species and populations from the whole North America continent to study the phylogenetic relationships of grape germplasm and their correlation with pierce’s disease resistance. This study paid much attention and works to evaluate disease resistance by quantifying the level of bacteria in stems and measuring Pierce’s disease symptoms on the canes and leaves. Results of 20 SSR markers to genotype 326 accessions of grape species well suggested a geographical divide in the southwest and southeastern US and Mexico. Two hundred sixty-six of these accessions, and an additional 12 PD resistant hybrid cultivars developed from southeastern US grape species, were evaluated for PD resistance. This work provide clear phylogenetic pattern for the grape genus in North America with interesting pierce’s disease resistance evaluation. But regretfully, no analyses were performed to test the correlation between this disease resistance trait and their phylogenetic and biogeographic relationships or explanation for the disease resistance pattern in North American grapes. Also, Results are too long and Discussion looks a little thin. Anyway, this study revealed an interesting phylogenetic pattern and the PD resistance pattern in North American grapes, and it is worthy to be accepted by the journal with some improvements

1. Phylogenetic history within Vitis is an interesting puzzle and topological relationships for the whole Vitis group including North America have been studied many times before. All these works should be compared and discussed in this study. Further, it is better to present a topological tree to show their phylogenetic relationships.

2. PD resistance data seems not to be well match phylogenetic divide of North American grapes, there is no clear east-west divide as shown in phylogenetic data. A correlation analyses should be used. Climate is a possible explanation for their different disease resistance, but there must have many other causes, such as from an evolutionary view.

3. Sample information including locations or sources for all the 346 accessions should be given in table or as a supplement.

4. To have a better presentations, subgroups in the two main groups should be also marked in Figure 2. If possible, hybridization zone and the Sierra Madre Oriental should also be noted on Figure 2 since they have been mentioned many times in the discussion.

5. Line 52, Muscadinia rotundifolia --> Vitis rotundifolia

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Reviewer #1: No

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Genetic analysis reveals an east-west divide within North American Vitis species that mirrors their resistance to Pierce's disease.

PONE-D-20-26579R1

Dear Dr. Walker,

We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements.

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Kind regards,

Tzen-Yuh Chiang

Academic Editor

PLOS ONE

Additional Editor Comments (optional):

Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. If the authors have adequately addressed your comments raised in a previous round of review and you feel that this manuscript is now acceptable for publication, you may indicate that here to bypass the “Comments to the Author” section, enter your conflict of interest statement in the “Confidential to Editor” section, and submit your "Accept" recommendation.

Reviewer #1: All comments have been addressed

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2. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Yes

**********

3. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

**********

4. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #1: Yes

**********

5. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.

Reviewer #1: Yes

**********

6. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: This manuscript has been largely and concisely revised though some minor comments were not accepted by the authors.

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7. PLOS authors have the option to publish the peer review history of their article (what does this mean?). If published, this will include your full peer review and any attached files.

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Reviewer #1: No