Peer Review History
Original SubmissionJune 24, 2020 |
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PONE-D-20-19501 Hypergravity as a Gravitational Therapy Mitigates the Effects of Knee Osteoarthritis on the Musculoskeletal System in a murine model PLOS ONE Dear Dr. Vico: Thank you for submitting your manuscript to PLOS ONE. After careful consideration, we feel that it has merit but does not fully meet PLOS ONE’s publication criteria as it currently stands. Therefore, we invite you to submit a revised version of the manuscript that addresses the points raised during the review process by an expertise reviewer and editorial comments as noted below. Please submit your revised manuscript by Oct 17 2020 11:59PM. If you will need more time than this to complete your revisions, please reply to this message or contact the journal office at plosone@plos.org. When you're ready to submit your revision, log on to https://www.editorialmanager.com/pone/ and select the 'Submissions Needing Revision' folder to locate your manuscript file. Please include the following items when submitting your revised manuscript:
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The PLOS ONE style templates can be found at https://journals.plos.org/plosone/s/file?id=wjVg/PLOSOne_formatting_sample_main_body.pdf and 2.We noticed you have some minor occurrence of overlapping text with previous publications, which needs to be addressed. In your revision ensure you cite all your sources (including your own works), and quote or rephrase any duplicated text outside the methods section. Further consideration is dependent on these concerns being addressed. Specific examples of the overlap include: *Introduction: sentence beginning "Patients experience pain... Daily activities...." - this overlaps considerably with: https://academic.oup.com/ptj/article/98/7/560/4998860 *Introduction: "Bone and muscle closely interact..." - this overlaps with: https://link.springer.com/article/10.1007/s00198-017-4151-8 *Introduction: "In others, exercise interventions...." - this overlaps with: https://www.oarsijournal.com/article/S1063-4584(18)30018-9/fulltext These are just some brief examples, there are other areas of text where the sentences/phrases appear in previously published works, but it's very important all submitted text is your own original writing. Additionally, please provide complete information about all efforts to minimize suffering peri- and post-operatively, including full analgesic regimen, supportive care and so forth. Please also state the mortality rate of the animals (if any) due to the experimental procedures. 3. Please include captions for your Supporting Information files at the end of your manuscript, and update any in-text citations to match accordingly. Please see our Supporting Information guidelines for more information: http://journals.plos.org/plosone/s/supporting-information. Additional Editor Comments (if provided): The authors show hypergravity in osteoarthritis mice (OA) has positive effects on trabecular bone and skeletal muscle typology, however negative effects on cortical bone. Specific comments to improve the manuscript are as follow: Methods-(p.5; line 109) verify the name correctly for mouse strain used ie., C57BL/6J. They can show Table 1: List of primers used for body mass index, bone and muscle genes in supplemental. Results-they can include a rationale to study bone and skeletal muscle parameters in osteoarthritis conditions. Figures-improve quality of all the figures and labeling. Supplemental material-please clarify taking a picture by Video “centri 2014.avi” or video display properly. [Note: HTML markup is below. Please do not edit.] Reviewers' comments: Reviewer's Responses to Questions Comments to the Author 1. Is the manuscript technically sound, and do the data support the conclusions? The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented. Reviewer #1: Yes ********** 2. Has the statistical analysis been performed appropriately and rigorously? Reviewer #1: Yes ********** 3. Have the authors made all data underlying the findings in their manuscript fully available? The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified. Reviewer #1: No ********** 4. Is the manuscript presented in an intelligible fashion and written in standard English? PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here. 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The manuscript by Dechaumet and colleagues entitled “Hypergravity as a Gravitational Therapy Mitigates the Effects of Knee Osteoarthritis on the Musculoskeletal System in a murine model” is an interesting paper exploring the impact on and possible application of chronic centrifugation in osteoarthritis. It is an interesting and well-prepared and executed study exploring the effects based on both histological and molecular data. General comments: Experiment set-up in centrifuge. The referred to S1 video was not accessible for review). The centrifuge was started and stopped a few times during the study. Also, in addition to data from reference 31: Please identify what the spin-up and spin-down time was for this experiment. Results: Body mass was unaffected by either condition: This is a surprising observation and should be addressed and discussed some more. In the work from ref 46 (Fuller et al.) In that paper from Fuller et al. there is a clear reduced body mass also at 2g as well as a difference in mass with wheel running while no changes are seen in this study. But also in Picquet et al (10.1152/ajpregu.00643.2001) there was a body weight decrease of 40% after 100 days at 2g, so quite a comparable set-up and, if I am not mistaken, performed in the same centrifuge. But seen in many other studies also in other species e.g. rabbit (see DOI: 10.1152/jappl.1978.45.1.51). Regarding body mass data: Please provide start values for this be as “Supplemental data”. In figure 6 there is an increase in adipocyte-related gene expression of C-EPBa and FAT/CD36 in Ctr 2g compared to Ctr 1g animals. CD36 has been linked to phenotypic features of the metabolic syndrome including insulin resistance and dyslipidemia, a phenomenon related to altered gravity. How does data from this study related to previous studies where hypergravity decreases fat related parameters; see e.g. Warren et al. J Appl Physiol 90: 606–614, 2001. Using lean and obese rats? Please elaborate in discussion. Minor comments: - L197: CV? Please explain. - L264: ‘since”? - L282: no changes found in functional tests (data not shown). However, I would propose to include the data as Supplemental material for future reference. Same for run wheel data (L341) - L317/L337: gravity should be with lower case ‘g’ - Fig. 1: Although quite common maybe good to explain ‘ROI’. - Fig. 1: In general, the images are of poor resolution which might be because of the journal conversion to pdf. Presume the originals are better - Fog. 1: Difference between gray and white (bone mineral / blood vessel) not very clear in a and c. c seems to be over exposed. Cortex nearly all white (and yellow?). - Fig 1: “of the Ctrl group”. What control group? Ctrl-1g or AO-1g? Please clarify - Fig. 2: “O/ Fast Green” should be “safranin O/Fast Green” - Fig. 2: Also, here identify the “Ctrl” better. - Fig. 4: Earth with capital ‘E’ ********** 6. PLOS authors have the option to publish the peer review history of their article (what does this mean?). If published, this will include your full peer review and any attached files. If you choose “no”, your identity will remain anonymous but your review may still be made public. Do you want your identity to be public for this peer review? For information about this choice, including consent withdrawal, please see our Privacy Policy. Reviewer #1: No [NOTE: If reviewer comments were submitted as an attachment file, they will be attached to this email and accessible via the submission site. Please log into your account, locate the manuscript record, and check for the action link "View Attachments". If this link does not appear, there are no attachment files.] While revising your submission, please upload your figure files to the Preflight Analysis and Conversion Engine (PACE) digital diagnostic tool, https://pacev2.apexcovantage.com/. PACE helps ensure that figures meet PLOS requirements. To use PACE, you must first register as a user. Registration is free. Then, login and navigate to the UPLOAD tab, where you will find detailed instructions on how to use the tool. If you encounter any issues or have any questions when using PACE, please email PLOS at figures@plos.org. Please note that Supporting Information files do not need this step. |
Revision 1 |
Hypergravity as a Gravitational Therapy Mitigates the Effects of Knee Osteoarthritis on the Musculoskeletal System in a murine model PONE-D-20-19501R1 Dear Dr. VICO, We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements. Within one week, you’ll receive an e-mail detailing the required amendments. When these have been addressed, you’ll receive a formal acceptance letter and your manuscript will be scheduled for publication. An invoice for payment will follow shortly after the formal acceptance. To ensure an efficient process, please log into Editorial Manager at http://www.editorialmanager.com/pone/, click the 'Update My Information' link at the top of the page, and double check that your user information is up-to-date. If you have any billing related questions, please contact our Author Billing department directly at authorbilling@plos.org. If your institution or institutions have a press office, please notify them about your upcoming paper to help maximize its impact. If they’ll be preparing press materials, please inform our press team as soon as possible -- no later than 48 hours after receiving the formal acceptance. Your manuscript will remain under strict press embargo until 2 pm Eastern Time on the date of publication. For more information, please contact onepress@plos.org. Kind regards, Dr. Sakamuri V. Reddy Academic Editor PLOS ONE Additional Editor Comments (optional): Reviewers' comments: |
Formally Accepted |
PONE-D-20-19501R1 Hypergravity as a Gravitational Therapy Mitigates the Effects of Knee Osteoarthritis on the Musculoskeletal System in a murine model Dear Dr. VICO: I'm pleased to inform you that your manuscript has been deemed suitable for publication in PLOS ONE. Congratulations! Your manuscript is now with our production department. If your institution or institutions have a press office, please let them know about your upcoming paper now to help maximize its impact. If they'll be preparing press materials, please inform our press team within the next 48 hours. Your manuscript will remain under strict press embargo until 2 pm Eastern Time on the date of publication. For more information please contact onepress@plos.org. If we can help with anything else, please email us at plosone@plos.org. Thank you for submitting your work to PLOS ONE and supporting open access. Kind regards, PLOS ONE Editorial Office Staff on behalf of Dr. Sakamuri V. Reddy Academic Editor PLOS ONE |
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