PONE-D-19-35094

Inventive nesting behaviour in the keyhole wasp Pachodynerus nasidens Latreille (Hymenoptera: Vespidae) in Australia, and the risk to aviation safety

PLOS ONE

Dear Alan House

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PLOS ONE

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Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Partly

Reviewer #2: Partly

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2. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: No

Reviewer #2: No

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3. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #1: No

Reviewer #2: No

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4. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.

Reviewer #1: Yes

Reviewer #2: Yes

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5. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: Dear Authors, I found your study very interesting and Ia also realize the urgency to publish this MS as it can help preventing accidents in airports.

I have made several comments and suggestions to enhance the quality of the MS, and I am especially concerned with the data and analyses performed. You have such rich data but they were not explored in its full magnitude. I also reommended several changes in the Methods section, to make the MS replicable.

Specific comments:

L 21 - 22 please rephase this sentence

Introducion - you need to work hard on your objectives and hypotheses (you can have hypotheses). As I read you results, I realized that you introduction (is good) lacks more robust objectives. You have investigated so many things, such as sex rations, influence of weather, environment and etc, and these factors are not presented in the Introduction.

L 57 - a parenthesis before Florida, but it does not close after Arizona (?)

L 163 - sample size = 12, correct? Or is there anymore treatments? How many probes per sheet? From your figures,

I counted 4 probes in one sheet and five probes in other sheet. Did you use all probe types (Table 1) in the experiment?

L 164 - incidents .... were suspected

Intercept traps - what is the sample size? For instance, you used 16 traps in airside and each trap consisted of 68 tubes, so in this

example you sample size is 68x16 = 1088 tubes? Is there another source of variation? Please provide these number in the MS, as it

helps to understand your statistical analyses. Moreover, you mention that tubes were replaced weekly, but it is not clear for how long.

Monitoring - here again I miss quantitative information.

"Probes were inspected weekly to monthly" - all probes? how many times were they inspected during the study period?

"Blocked probes were removed from the panels" - were these probes replaced by now ones?

Data Analysis - I think you should explore the data with the following tests. Please check them to see if they are appropriate to the data you collected.

I think using chi-squared tests are a poor indicator of statistical power.

lmm, glmm, or glmmTMB = number of occupied probes ~ location (4 locations) + probe size/volume/apperture + probe height + weather + time + (1|panels)

lm or Anova = occupied traps ~ location (2 locations) + time

L 196 - 201 - this shoud be on Methods

L 313. a dot is missing after P (wasp genus)

L 202 - I did not understand which variables you used in this multiple regression. What as the intercept, the number of wasps?

Results

L 208 - only here I see the duration of the study. I did not understand this number of 93 blocked probes. In the Methods section,

you need to provide more information on sample sizes. 93 probes correspond to what percentage of probes available? Here I see as well that there is

a time variable, and also a weather variable. From what you presented here, I cannot reverse the results to replicate a methodology.

Figure 3 - I suggest presenting the data in percent values, because you mention in the MS that you did not have a perfectly balanced design

L 231 - 233 - There must be problems with this Anova. I suggest changing the test (as I recommended above)

Figure 4 - these relations are not linear, and in Fig 4a there is an outlier in the data. Moreover, the text displays a value o R = 0.73, e the figure it is 0.53

Reviewer #2: This is an interesting study on the nesting ecology of a solitary wasp, Pachodynerus nasidens (Eumeninae). The authors made an innovative experiment based on a real problem frequently observed in airports. The study is important since provides the natural history aspects of the wasp and explores the preference of nesting sites which are the aircraft model pitots. The results are well explored but they used parametric ANOVA, so it is to state whether samples have normal distribution or may they transformed the raw data. Also, the data are not available.

The authors may suggest a further comment for companies to modify size or shape(?) of pitots since they showed a preference of probe aperture diameter between 4-7.2 mm.

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Reviewer #1: No

Reviewer #2: No

[NOTE: If reviewer comments were submitted as an attachment file, they will be attached to this email and accessible via the submission site. Please log into your account, locate the manuscript record, and check for the action link "View Attachments". If this link does not appear, there are no attachment files to be viewed.]

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PONE-D-19-35094R1

Inventive nesting behaviour in the keyhole wasp Pachodynerus nasidens Latreille (Hymenoptera: Vespidae) in Australia, and the risk to aviation safety

PLOS ONE

Dear Alan PN House

Thank you for submitting your manuscript to PLOS ONE. After careful consideration, we feel that it has merit but does not fully meet PLOS ONE’s publication criteria as it currently stands. Therefore, we invite you to submit a revised version of the manuscript that addresses the points raised during the review process.

We would appreciate receiving your revised manuscript by 20 days. When you are ready to submit your revision, log on to https://www.editorialmanager.com/pone/ and select the 'Submissions Needing Revision' folder to locate your manuscript file.

If you would like to make changes to your financial disclosure, please include your updated statement in your cover letter.

To enhance the reproducibility of your results, we recommend that if applicable you deposit your laboratory protocols in protocols.io, where a protocol can be assigned its own identifier (DOI) such that it can be cited independently in the future. For instructions see: http://journals.plos.org/plosone/s/submission-guidelines#loc-laboratory-protocols

Please include the following items when submitting your revised manuscript:

• A rebuttal letter that responds to each point raised by the academic editor and reviewer(s). This letter should be uploaded as separate file and labeled 'Response to Reviewers'.
• A marked-up copy of your manuscript that highlights changes made to the original version. This file should be uploaded as separate file and labeled 'Revised Manuscript with Track Changes'.
• An unmarked version of your revised paper without tracked changes. This file should be uploaded as separate file and labeled 'Manuscript'.

Please note while forming your response, if your article is accepted, you may have the opportunity to make the peer review history publicly available. The record will include editor decision letters (with reviews) and your responses to reviewer comments. If eligible, we will contact you to opt in or out.

We look forward to receiving your revised manuscript.

Kind regards,

Kleber Del-Claro, PhD

Academic Editor

PLOS ONE

Additional Editor Comments (if provided):

Dear Author, our colleagues are delaying a lot in reviews mainly due to the crazy times we are living. Please, do all the modifications that the reviewer asked you and I will take the final decision without new round.

Best, wishes,

Kleber

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Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. If the authors have adequately addressed your comments raised in a previous round of review and you feel that this manuscript is now acceptable for publication, you may indicate that here to bypass the “Comments to the Author” section, enter your conflict of interest statement in the “Confidential to Editor” section, and submit your "Accept" recommendation.

Reviewer #1: All comments have been addressed

**********

2. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Yes

**********

3. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

**********

4. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #1: No

**********

5. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.

Reviewer #1: Yes

**********

6. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: Dear authors, I appreciate a lot the polite responses reagarding the first row of peer review. I still have some minor doubts/suggestions:

Please cite the R packages used in the study

How did you collecte the data of climatic variables?

Why including location as a random variable? I believe this is a fixed factor

L 234 - I did not understand the "Monte Carlo sample size". Is this misplaced?

L 244 - 249 and Table 2 - these are Methods (above) and not Data Analyses

L 261 - 264 - please prove the R² values

L 281 - The mixed effects model confirmed probe type was not significant but then you mention that they were significant.

This is a bit confusing. I suggest stating that probes were significant, because above you also show that probes > 5 mm were found to have nests

L 351 - 354 - are these r² values based on which test? Did you perform a regression here or is this part of the sex ratio test (Cochran-Armitage test). IF you are having difficulties to express the data collection and analyses, I suggest makibg a table like Table 1 (with you adaptations) made in the following papers. It helps a lot to understand the tests and figures associated with each objective.

1 - Coverdale, T. C., Goheen, J. R., Palmer, T. M., & Pringle, R. M. (2018). Good neighbors make good defenses: associational refuges reduce defense investment in African savanna plants. Ecology, 99(8), 1724-1736.

2 - Ancco Valdivia, F. G., Alves-Silva, E., & Del‐Claro, K. (2020). Differences in size and energy content affect the territorial status and mating success of a neotropical dragonfly. Austral Ecology.

Figure 4a - there is no need for this Figure

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7. PLOS authors have the option to publish the peer review history of their article (what does this mean?). If published, this will include your full peer review and any attached files.

If you choose “no”, your identity will remain anonymous but your review may still be made public.

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Reviewer #1: No

[NOTE: If reviewer comments were submitted as an attachment file, they will be attached to this email and accessible via the submission site. Please log into your account, locate the manuscript record, and check for the action link "View Attachments". If this link does not appear, there are no attachment files to be viewed.]

While revising your submission, please upload your figure files to the Preflight Analysis and Conversion Engine (PACE) digital diagnostic tool, https://pacev2.apexcovantage.com/. PACE helps ensure that figures meet PLOS requirements. To use PACE, you must first register as a user. Registration is free. Then, login and navigate to the UPLOAD tab, where you will find detailed instructions on how to use the tool. If you encounter any issues or have any questions when using PACE, please email us at figures@plos.org. Please note that Supporting Information files do not need this step.

Inventive nesting behaviour in the keyhole wasp Pachodynerus nasidens Latreille (Hymenoptera: Vespidae) in Australia, and the risk to aviation safety

PONE-D-19-35094R2

Dear Dr. Alan PN House,

We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements.

Within one week, you’ll receive an e-mail detailing the required amendments. When these have been addressed, you’ll receive a formal acceptance letter and your manuscript will be scheduled for publication.

An invoice for payment will follow shortly after the formal acceptance. To ensure an efficient process, please log into Editorial Manager at http://www.editorialmanager.com/pone/, click the 'Update My Information' link at the top of the page, and double check that your user information is up-to-date. If you have any billing related questions, please contact our Author Billing department directly at authorbilling@plos.org.

If your institution or institutions have a press office, please notify them about your upcoming paper to help maximize its impact. If they’ll be preparing press materials, please inform our press team as soon as possible -- no later than 48 hours after receiving the formal acceptance. Your manuscript will remain under strict press embargo until 2 pm Eastern Time on the date of publication. For more information, please contact onepress@plos.org.

Kind regards,

Kleber Del-Claro, PhD

Academic Editor

PLOS ONE

Additional Editor Comments (optional):

Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. If the authors have adequately addressed your comments raised in a previous round of review and you feel that this manuscript is now acceptable for publication, you may indicate that here to bypass the “Comments to the Author” section, enter your conflict of interest statement in the “Confidential to Editor” section, and submit your "Accept" recommendation.

Reviewer #1: All comments have been addressed

**********

2. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Yes

**********

3. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

**********

4. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #1: Yes

**********

5. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.

Reviewer #1: Yes

**********

6. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: All suggestions were addressed by the authors. I believe the MS is resy for publication in its current form

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7. PLOS authors have the option to publish the peer review history of their article (what does this mean?). If published, this will include your full peer review and any attached files.

If you choose “no”, your identity will remain anonymous but your review may still be made public.

Do you want your identity to be public for this peer review? For information about this choice, including consent withdrawal, please see our Privacy Policy.

Reviewer #1: No