PONE-D-20-32626

Insect wing extract: A novel source for green synthesis of nanoparticles of antioxidant and antimicrobial potential

PLOS ONE

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Pradeep Kumar

Academic Editor

PLOS ONE

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Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Yes

Reviewer #2: Partly

Reviewer #3: Yes

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2. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: I Don't Know

Reviewer #2: I Don't Know

Reviewer #3: N/A

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3. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #1: Yes

Reviewer #2: Yes

Reviewer #3: Yes

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4. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.

Reviewer #1: Yes

Reviewer #2: Yes

Reviewer #3: Yes

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5. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: Dear authors,

Great congratulations on publishing this high-quality paper. There are some points should be considered in the final revision of this work.

1-Why the authors select this insect for synthesis of target particles?

2-What is the benefit of applied method in this study?

3-AgNPs are one of the sources of ROS production. How these particles may show such discussed benefits and scavenge free radicals. The provided proofs for this section of experimental assays is not acceptable and the authors must develop their idea regarding the antioxidant activity of AgNPs.

4-For what purpose these particles were synthesized? Would you please kindly discuss the clinical or industrial application of the synthesized particles regarding the method you applied in this study.

5-The authors should determine the synthesized particles are not toxic for human body. Please use MTT assay to determine the potential toxicity effects of these particles for living cells.

6-Figures were not prepared at high-resolution pixels. Please modify the source of figures to provide at least high-quality images for respective readers.

7-Please provide the screenshots of fungal assays as supplementary material. The effectiveness of synthesized particles for inhibition of fungal or bacterial growth on lab plates should be provided.

8-Please discuss the industrial application of the methodology of this paper plus its possible dangers for nearby environment.

Reviewer #2: Dear Author

The manuscript entitled "Insect wing extract: A novel source for green synthesis of nanoparticles of antioxidant and antimicrobial potential" looks interesting and needs to address following queries before considering for publication

Overall Query: Authors needs to justify the importance of insect wings as mentioned by authors "abundantly available and rich in proteins, polysaccharides and lipids" in introduction section. Need to add some imprtant findings and how the nanoparticles synthesized from targeted source will be better than available sources.

Secondaly authors can compare the bioactivities in normal extracts and nanoparticles that will also be interesting.

Minor corrections:

Title : Should be revised as

Silver nanoparticles from Insect wing extract : Biosynthesis and evaluation for antioxidant and antimicrobial potential

2)Abstract

Results of antioxidant and antimicrobial activity need to be mentioned

3)Introduction

Need details various source of biosynthesis of NPs and antioxidant and antimicrobial properties of AgNPs

4)Materials and Methods

Characterization of ------

Brief details of each method should be added

5)Statistical analysis

Cite some reference and source of software

6)Results and Discussion

Figure 4a and 5a and 5b can be given as supplementary figures

7)References

More recent and relevant references can be added

Reviewer #3: This is in reference to the manuscript entitled “Insect wing extract: A novel source for green synthesis of nanoparticles of antioxidant and antimicrobial potential (PONE-D-20-32626) submitted for publication in PLOS ONE journal.

The authors have presented an interesting study concerning green synthesis of nanoparticles. The topic has a novel approach. This is a very valid and interesting study, which can contribute to the body of knowledge. The paper is well-organized. The writing was sufficiently informative to deliver the message of the manuscript. A praiseworthy study was performed by the authors giving sufficient information on producing silver nanoparticles from insect wings.

Hence, I would suggest accepting the paper with minor corrections.

Mention the importance of some significant results obtained in the study in the conclusion section with an emphasis on its future applications.

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Do you want your identity to be public for this peer review? For information about this choice, including consent withdrawal, please see our Privacy Policy.

Reviewer #1: Yes: Hassan Rasouli

Reviewer #2: No

Reviewer #3: No

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Silver nanoparticles from Insect wing extract : Biosynthesis and evaluation for antioxidant and antimicrobial potential

PONE-D-20-32626R1

Dear Dr. Bee,

We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements.

Within one week, you’ll receive an e-mail detailing the required amendments. When these have been addressed, you’ll receive a formal acceptance letter and your manuscript will be scheduled for publication.

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Kind regards,

Pradeep Kumar

Academic Editor

PLOS ONE

Additional Editor Comments (optional):

Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. If the authors have adequately addressed your comments raised in a previous round of review and you feel that this manuscript is now acceptable for publication, you may indicate that here to bypass the “Comments to the Author” section, enter your conflict of interest statement in the “Confidential to Editor” section, and submit your "Accept" recommendation.

Reviewer #1: All comments have been addressed

Reviewer #2: All comments have been addressed

Reviewer #3: All comments have been addressed

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2. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Yes

Reviewer #2: Yes

Reviewer #3: Yes

**********

3. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

Reviewer #2: Yes

Reviewer #3: Yes

**********

4. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #1: Yes

Reviewer #2: Yes

Reviewer #3: Yes

**********

5. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.

Reviewer #1: Yes

Reviewer #2: Yes

Reviewer #3: Yes

**********

6. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: Dear Authors,

Thank you very much for revision of your paper. I've check the content of this paper and it was improved precisely. I have no further comments for evaluation. The manuscript is now ready for publication.

Reviewer #2: Dear Authors

The manuscript is been improved and the authors have addressed all comments given by the reviewers and editors.

Regards

Reviewer #3: The authors have well addressed all the comments. Hence, the article should be considered for publication.

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7. PLOS authors have the option to publish the peer review history of their article (what does this mean?). If published, this will include your full peer review and any attached files.

If you choose “no”, your identity will remain anonymous but your review may still be made public.

Do you want your identity to be public for this peer review? For information about this choice, including consent withdrawal, please see our Privacy Policy.

Reviewer #1: Yes: Hassan Rasouli

Reviewer #2: No

Reviewer #3: No