PONE-D-20-17370

Difference in pyruvic acid metabolism between neonatal and adult mouse lungs exposed to hyperoxia

PLOS ONE

Dear Dr. Fumihiko Namba,

Thank you for submitting your manuscript to PLOS ONE. After careful consideration, we feel that it has merit but does not fully meet PLOS ONE’s publication criteria as it currently stands. Therefore, we invite you to submit a revised version of the manuscript that addresses the points raised during the review process.

Though it is a revised submission, it has been assigned to new editor with a new manuscript number due to the fact that the revision has not been submitted on time. I could see reviewer comments and your revised version of the manuscript with all your modifications and responses. For a strong third opinion, I have sent it to another reviewer, who had revised it and asked for minor revision. 

I would be thankful to you if you could revise the manuscript and submit it for consideration. 

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We look forward to receiving your revised manuscript.

Kind regards,

Ch Ratnasekhar, Ph.D.

Academic Editor

PLOS ONE

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Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Yes

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2. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

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Reviewer #1: Yes

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Reviewer #1: Yes

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5. Review Comments to the Author

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Reviewer #1: The reviewed study entitled: “Difference in pyruvic acid metabolism between

neonatal and adult mouse lungs exposed to hyperoxia” by Tanaka et al. used CE TOFMS to

investigate the metabolic changes between hyperoxia in neonatal and adult mice. The analytical methods are sound and very well described in the manuscript. This is an interesting study and technique and the results will provide valuable information into using neonatal mice to investigate metabolic changes associated with respiratory exposures and disease.

Minor Comments:

1. In the final paragraph of the introduction on page three, the author should include some references on using CE TOFMS as an analytical tool for metabolomics. This would validate the technique and inform the readers on the how the technique was previously used.

2. Were any of the metabolites confirmed through MS/MS analysis combined with spectral library searching, or were they identified based on intact mass and electrophoresis? If they were only identified based on these 2 parameters, the author should include some information on the mass accuracy of the instrumentation used for the analysis and the mass error for the metabolite in parts per million (ppm).

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Reviewer #1: No

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Difference in pyruvic acid metabolism between neonatal and adult mouse lungs exposed to hyperoxia

PONE-D-20-17370R1

Dear Dr. Namba,

We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements.

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Kind regards,

Ch Ratnasekhar, Ph.D.

Academic Editor

PLOS ONE

Additional Editor Comments (optional):

Reviewers' comments: