PONE-D-20-12129

Phylogeny and species delimitation of the genus Longgenacris and Fruhstorferiola viridifemorata species group (Orthoptera: Acrididae: Melanoplinae) based on molecular evidence

PLOS ONE

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Kind regards,

Feng ZHANG, Ph.D.

Academic Editor

PLOS ONE

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Reviewers' comments:

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Comments to the Author

1. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Yes

Reviewer #2: Yes

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2. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

Reviewer #2: Yes

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Reviewer #1: Yes

Reviewer #2: Yes

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4. Is the manuscript presented in an intelligible fashion and written in standard English?

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Reviewer #1: Yes

Reviewer #2: No

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5. Review Comments to the Author

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Reviewer #1: The content of this manuscript is quite good. For phylogeny reconstruction, I don't think use Tetrigidae and Tettigoniidae as outgroups is right choice. Furthermore, I hope the author adds Bayesian inference analysis. The quality of English needs improving.

Other minor points are:

line 36 Delete Keyword “Longgenacris" or "Longgenacris rufiantennus";

line 104 "cytochrome c oxidase subunit I (COI, cox1)"→"COI or cox1";

line 122 "100% ethanol"→"anhydrous ethanol";

line 140-141 "PCR products were sent to the biological company", Which company？

line 183 "TSC1.21"→"TCS1.21";

Reviewer #2: This article deserves publication. However, there are the following problems.

1) Normally, as for the raltionship between species and above, NJ tree is not optimal selection.

2) On the page 16, line 403, “ITS region had a much lower evolution rate than COI gene in our datasets”, I’m very interested in it. Can the author explain this?

3) All figures are not clear. Low photo resolution.

4) English speakers are requested to polish this draft.

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Reviewer #1: No

Reviewer #2: No

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Attachments

Attachment

Submitted filename: PONE-D-20-12129_reviewer.pdf

Phylogeny and species delimitation of the genus Longgenacris and Fruhstorferiola viridifemorata species group (Orthoptera: Acrididae: Melanoplinae) based on molecular evidence

PONE-D-20-12129R1

Dear Dr. Huang,

We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements.

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Kind regards,

Feng ZHANG, Ph.D.

Academic Editor

PLOS ONE

Additional Editor Comments (optional):

Reviewers' comments: