Peer Review History
Original SubmissionMarch 20, 2020 |
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PONE-D-20-08055 Unravelling the changes during induced vitellogenesis in female European eel through RNA-Seq: what happens to the liver? PLOS ONE Dear Dr Bertolini, Thank you for submitting your manuscript to PLOS ONE. After careful consideration, we feel that it has merit but does not fully meet PLOS ONE’s publication criteria as it currently stands. Therefore, we invite you to submit a revised version of the manuscript that addresses the points raised during the review process. Your manuscript has been reviewed by two referees who are recognized experts in this field. Both reviewers have made several comments that you will have to consider in order to revise your manuscript accordingly. We would appreciate receiving your revised manuscript by Jun 13 2020 11:59PM. When you are ready to submit your revision, log on to https://www.editorialmanager.com/pone/ and select the 'Submissions Needing Revision' folder to locate your manuscript file. If you would like to make changes to your financial disclosure, please include your updated statement in your cover letter. To enhance the reproducibility of your results, we recommend that if applicable you deposit your laboratory protocols in protocols.io, where a protocol can be assigned its own identifier (DOI) such that it can be cited independently in the future. For instructions see: http://journals.plos.org/plosone/s/submission-guidelines#loc-laboratory-protocols Please include the following items when submitting your revised manuscript:
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Please remove any funding-related text from the manuscript and let us know how you would like to update your Funding Statement. Currently, your Funding Statement reads as follows: 'The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.' Additional Editor Comments (if provided): [Note: HTML markup is below. Please do not edit.] Reviewers' comments: Reviewer's Responses to Questions Comments to the Author 1. Is the manuscript technically sound, and do the data support the conclusions? The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented. Reviewer #1: Yes Reviewer #2: Yes ********** 2. Has the statistical analysis been performed appropriately and rigorously? Reviewer #1: Yes Reviewer #2: Yes ********** 3. Have the authors made all data underlying the findings in their manuscript fully available? The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified. Reviewer #1: Yes Reviewer #2: Yes ********** 4. Is the manuscript presented in an intelligible fashion and written in standard English? PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here. Reviewer #1: Yes Reviewer #2: Yes ********** 5. Review Comments to the Author Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters) Reviewer #1: The manuscript describes a study on European eel vitellogenesis with a RNA sequencing approach, making a differential expression analysis of liver samples from yellow and silvering female eels. In general, the experimental design and methodology seems good, the manuscript is well written, and the discussed ideas are relevant (at least considering the grade of hypothetical conclusions required for this type of results). I included a few suggestions to be considered by the authors, and found some formal mistakes that should be corrected (especially references format!). Introduction L74. migration instead of migrating? L76. 80´s or even before in some places L87 Reference 18. For a more specific information on eel, you could use Mylonas et al., 2017 (http://dx.doi.org/10.1016/j.aquaculture.2016.04.021). L101 (plus comments on L276 and L394). There is scarce information available on the enzymatic control of fatty acid biosynthesis in the eel. Baeza et al., 2014´s (http://dx.doi.org/10.1016/j.aquaculture.2014.03.045) and 2015´s studies (http://dx.doi.org/10.1016/j.cbpa.2014.11.022) played with the idea of a liver involved in the lipids mobilization from muscle to gonad during gonadal maturation, but also evidenced de novo synthesis of lipids in the liver (in especial palmitic acid and EPA). Can your results clarify something on the role of European eel fatty desaturases and elongases?, and maybe relating them with dietary necessities for this species? L129, L142, L205. 20 ºC (separate number and unit) L134. Why fish were pit-tagged? L137. S.L.U. L166. 150 bp Results L224. Maybe is better to talk of a 1.6 fold increase of HSI. L228. Vitellogenic L234. 57% L249. ...in the pre-spawning samples. L250. (VTG1 and VTG2) L298. Aorta mention seems too specific. Vascular tissues?? Discussion L330. ...and used for ovary development and egg yolk... L356. Check the recent paper from Morini et al., 2020. (http://dx.doi.org/10.1017/S1751731119003355). In the European eel, up-regulation of VTG under estradiol treatment was observed in eel in vivo (Burzawa-Gérard and Dumas-Vidal, 1991) as well as in vitro by hepatocyte primary cultures (Lafont et al., 2016). Nevertheless, ovaries from E2-treated eels are not able to incorporate Vtg (Dufour et al., 1988), and CPE treatment is necessary to induce ovarian vitellogenesis (Mazzeo et al., 2014). They found found that vtgr transcription already occurs during early PV of immature eel and is not further activated in vitellogenic oocytes. Can your (liver) data support this conclusion somehow? L364. chemical disruptors? L371. Morini et al. 2017 (http://dx.doi.org/10.1016/j.cbpa.2017.02.009) reported the expression of several progestin receptors in the liver of immature eels, and described the changes of the expression of these genes happening in the testes during hormonally-induced spermatogenesis. Does your data suggest differential expression of these genes in the liver induced by hormonal treatment in the females?. References In general, follow the journal´s format. Full references (including volume and page numbering, doi and PMID) are required. Write especies´s name in italics. Avoid writing with capitals every word of the titles. Delete “and” from the list of authors (limited to 6; rest: et al.). Check abbreviated journal names. L533. IUCN? L550, 563. European L572. It is still under review?. Add full reference or delete. L662. Oncorhynchus L681. Nutritional regulation of insulin-like growth factor-I mRNA expression in salmon tissues??? Reviewer #2: The experiments were well designed in the study. In my opinion, the findings based on genes included in biological processes of the study will provide new insights for vitellogenesis in the eel or reproductive studies to be considered in other fish species. However, authors should take attention some issues regarding manuscript content. Authors should make corrections by controlling the text carefully according to the journal's rules. Some mistakes, for example in line 122, the length of the fish (76 ± 136 3 cm) should be corrected. A representative figure taken from the gonadal sections (especially vitellogenic stage) pertained to week 9 should be inserted in the results with a control section (previtellogenic stage). ********** 6. PLOS authors have the option to publish the peer review history of their article (what does this mean?). If published, this will include your full peer review and any attached files. If you choose “no”, your identity will remain anonymous but your review may still be made public. Do you want your identity to be public for this peer review? For information about this choice, including consent withdrawal, please see our Privacy Policy. Reviewer #1: Yes: Juan F. Asturiano Reviewer #2: No [NOTE: If reviewer comments were submitted as an attachment file, they will be attached to this email and accessible via the submission site. Please log into your account, locate the manuscript record, and check for the action link "View Attachments". If this link does not appear, there are no attachment files to be viewed.] While revising your submission, please upload your figure files to the Preflight Analysis and Conversion Engine (PACE) digital diagnostic tool, https://pacev2.apexcovantage.com/. PACE helps ensure that figures meet PLOS requirements. To use PACE, you must first register as a user. Registration is free. Then, login and navigate to the UPLOAD tab, where you will find detailed instructions on how to use the tool. If you encounter any issues or have any questions when using PACE, please email us at figures@plos.org. Please note that Supporting Information files do not need this step. |
Revision 1 |
Unravelling the changes during induced vitellogenesis in female European eel through RNA-Seq: what happens to the liver? PONE-D-20-08055R1 Dear Dr. Bertolini, We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements. Within one week, you’ll receive an e-mail detailing the required amendments. When these have been addressed, you’ll receive a formal acceptance letter and your manuscript will be scheduled for publication. An invoice for payment will follow shortly after the formal acceptance. To ensure an efficient process, please log into Editorial Manager at http://www.editorialmanager.com/pone/, click the 'Update My Information' link at the top of the page, and double check that your user information is up-to-date. If you have any billing related questions, please contact our Author Billing department directly at authorbilling@plos.org. If your institution or institutions have a press office, please notify them about your upcoming paper to help maximize its impact. If they’ll be preparing press materials, please inform our press team as soon as possible -- no later than 48 hours after receiving the formal acceptance. Your manuscript will remain under strict press embargo until 2 pm Eastern Time on the date of publication. For more information, please contact onepress@plos.org. Kind regards, Hubert Vaudry Academic Editor PLOS ONE Additional Editor Comments (optional): Reviewers' comments: Reviewer's Responses to Questions Comments to the Author 1. If the authors have adequately addressed your comments raised in a previous round of review and you feel that this manuscript is now acceptable for publication, you may indicate that here to bypass the “Comments to the Author” section, enter your conflict of interest statement in the “Confidential to Editor” section, and submit your "Accept" recommendation. Reviewer #1: All comments have been addressed Reviewer #2: All comments have been addressed ********** 2. Is the manuscript technically sound, and do the data support the conclusions? The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented. Reviewer #1: Yes Reviewer #2: Yes ********** 3. Has the statistical analysis been performed appropriately and rigorously? Reviewer #1: Yes Reviewer #2: Yes ********** 4. Have the authors made all data underlying the findings in their manuscript fully available? The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified. Reviewer #1: Yes Reviewer #2: Yes ********** 5. Is the manuscript presented in an intelligible fashion and written in standard English? PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here. Reviewer #1: Yes Reviewer #2: Yes ********** 6. Review Comments to the Author Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters) Reviewer #1: This second version reflects all the comments ans suggestions I previously did. In my opinion, the manuscript is ready to be published. Reviewer #2: All requested recommendations have been completed by authors. The revised manuscript can be accepted for publication. ********** 7. PLOS authors have the option to publish the peer review history of their article (what does this mean?). If published, this will include your full peer review and any attached files. If you choose “no”, your identity will remain anonymous but your review may still be made public. Do you want your identity to be public for this peer review? For information about this choice, including consent withdrawal, please see our Privacy Policy. Reviewer #1: Yes: Juan F. Asturiano Reviewer #2: No |
Formally Accepted |
PONE-D-20-08055R1 Unravelling the changes during induced vitellogenesis in female European eel through RNA-Seq: what happens to the liver? Dear Dr. Bertolini: I'm pleased to inform you that your manuscript has been deemed suitable for publication in PLOS ONE. Congratulations! Your manuscript is now with our production department. If your institution or institutions have a press office, please let them know about your upcoming paper now to help maximize its impact. If they'll be preparing press materials, please inform our press team within the next 48 hours. Your manuscript will remain under strict press embargo until 2 pm Eastern Time on the date of publication. For more information please contact onepress@plos.org. If we can help with anything else, please email us at plosone@plos.org. Thank you for submitting your work to PLOS ONE and supporting open access. Kind regards, PLOS ONE Editorial Office Staff on behalf of Dr. Hubert Vaudry Academic Editor PLOS ONE |
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