PONE-D-20-03673

Genetic characterization of a new radish introgression line carrying the restorer gene for Ogura CMS in Brassica napus

PLOS ONE

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Yong Pyo Lim

Academic Editor

PLOS ONE

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Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Yes

Reviewer #2: Yes

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2. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: N/A

Reviewer #2: Yes

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3. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #1: Yes

Reviewer #2: Yes

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4. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.

Reviewer #1: Yes

Reviewer #2: Yes

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5. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: In their study, Guo et al. present an investigation into the Rfo introgression in CLR6430. Unlike other restorer line, they find Rfo on N19 (C9?) in a differently sized introgression.

I can't find any fault with the general outline of the study or the findings, only minor things (below).

It seems that the short reads generated in this study are openly available, however, the genome assembly generated is not. Will the authors deposit this assembly somewhere?

Some minor things:

Line 29, typo, 'introgresssed'

Line 170 - the link in the PDF is broken and points to 'http://wsmartins.net/websat/ with default', should be without the 'with default'

Line 218 - incomplete sentence? 'Restorer gene was detected by FISH using C genome and BAC fish probe while A genome of B. napus as block'

Line 233, Table 3 - typo, 'Cleaned eads'

Line 265, why is Baiyuchun mentioned only once here? Why is it not in the methods?

Line 287, typo - 'Raphnobrassica'

There are a few more smaller typos which should have been caught by rigorous spell checking.

Reviewer #2: This manuscript 'Genetic characterization of a new radish introgression line carrying the restorer gene

for Ogura CMS in Brassica napus' is based on well defined problem and conducted in a profound way. It is a significant improvement from the earlier available studies. This should be accepted for publication after incorporating the following corrections:

i. Introduction: Explain the importance of restores in oilseed brassicas

ii. In the materials and method, the population developed using the CMS lines and restores and maintainers with restorers were mentioned. However, it has not been made clear how these population were used for this study. The method used in Table 5 is not mentioned clearly in this section.

iii. Results: Explained appropriately, however, it is difficult to understand how the different populations were used in validation of the designed markers and segregation ratio. Explain these portion elaborately.

iv. Discussion: This section need improvement in terms of the works done and its significance. This part should explain all the experiments conducted supported by sufficient number of citations.

Comments in the manuscript is also attached for further refernce.

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Reviewer #1: No

Reviewer #2: Yes: Dr. Shyam Sundar Dey

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Attachments

Attachment

Submitted filename: PONE-D-20-03673_reviewer.pdf

Genetic characterization of a new radish introgression line carrying the restorer gene for Ogura CMS in Brassica napus

PONE-D-20-03673R1

Dear Dr. Guo,

We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements.

Within one week, you’ll receive an e-mail detailing the required amendments. When these have been addressed, you’ll receive a formal acceptance letter and your manuscript will be scheduled for publication.

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Kind regards,

Yong Pyo Lim

Academic Editor

PLOS ONE

Additional Editor Comments (optional):

Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. If the authors have adequately addressed your comments raised in a previous round of review and you feel that this manuscript is now acceptable for publication, you may indicate that here to bypass the “Comments to the Author” section, enter your conflict of interest statement in the “Confidential to Editor” section, and submit your "Accept" recommendation.

Reviewer #1: All comments have been addressed

Reviewer #2: All comments have been addressed

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2. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Yes

Reviewer #2: Yes

**********

3. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: N/A

Reviewer #2: Yes

**********

4. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #1: Yes

Reviewer #2: Yes

**********

5. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.

Reviewer #1: Yes

Reviewer #2: Yes

**********

6. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: I'm happy with the way my recommendations were implemented. The data has been added to SRA and typos have been fixed. Thanks!

Reviewer #2: The manuscript entitled, ' Genetic characterization of a new radish introgression line carrying the restorer gene for Ogura CMS in Brassica napus , has been improved and revised as per the suggestions of the reviewers. Therefore, in the present format it can be accepted for publication.

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7. PLOS authors have the option to publish the peer review history of their article (what does this mean?). If published, this will include your full peer review and any attached files.

If you choose “no”, your identity will remain anonymous but your review may still be made public.

Do you want your identity to be public for this peer review? For information about this choice, including consent withdrawal, please see our Privacy Policy.

Reviewer #1: No

Reviewer #2: Yes: Dr. Shyam Sundar Dey