PONE-D-20-18913

Evaluating the appropriate oral lipid tolerance test model for investigating plasma triglyceride elevation in mice

PLOS ONE

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Ouliana Ziouzenkova, PhD

Academic Editor

PLOS ONE

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Reviewers' comments:

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Comments to the Author

1. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Yes

Reviewer #2: Yes

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2. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

Reviewer #2: Yes

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3. Have the authors made all data underlying the findings in their manuscript fully available?

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Reviewer #1: Yes

Reviewer #2: Yes

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4. Is the manuscript presented in an intelligible fashion and written in standard English?

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Reviewer #1: Yes

Reviewer #2: Yes

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5. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: Authors evaluated rodent models for OLTT and then suggested an optimal method.

Basically, the reported models would be fine because pharmacological substances would always be evaluated compared to controls. However, the larger the dynamic rage, the clearer the results from the bioassay. OTLL models were evaluated under a variety of conditions, and the suggested method could be helpful to researchers.

The publication of this manuscript in PLOS ONE can be supported after the suitable modification as follows.

-Abstract need to be concise and written shorter.

-Page 7, line 103, “I selected….” should be rewritten. Do not use “I”.

-All figures should be provided as high-resolution images.

-Mark statistical significance on the graph.

-In figure 2, please make it easy to distinguish.

Reviewer #2: The manuscript entitled “Evaluating the appropriate oral lipid tolerance test model for investigating plasma triglyceride elevation in mice” have described the complete standardization of a novel model to evaluate the postprandial hyperlipidemia trough oral lipid tolerance test, demonstrating which is the best strain, the optimum oil and the difference between gender...

The study was written carefully and well in terms of language. However, this work requires minor corrections.

Minor issues:

- Ethically it is an excellent idea to use the same animals for different experiments, however, in this case a method is being standardized and the age must always be the same to be exact. I suggest that in the future this be done with animals of the same age in order to confirm the results obtained in your model.

- In the high-performance thin-layer chromatography plate section in line 169-172 i suggest to mention which standard was used to compare and determine the lipids.

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Reviewer #1: No

Reviewer #2: No

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Evaluating the appropriate oral lipid tolerance test model for investigating plasma triglyceride elevation in mice

PONE-D-20-18913R1

Dear Dr. Ochiai,

We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements.

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Kind regards,

Ouliana Ziouzenkova, PhD

Academic Editor

PLOS ONE