PONE-D-19-31278

Gene Polymorphisms of Patatin-like phospholipase domain containing 3 (PNPLA3), Adiponectin, Leptin in Diabetic Obese Patients

PLOS ONE

Dear Dr Hafez,

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Kind regards,

Nadia M Hamdy, Ph.D.

Academic Editor

PLOS ONE

Additional Editor Comments:

Dear

English language needs revision and paragraphs need rephrasing as well

More details are required in the methods

Limitations and future prospective are to be there

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Attachments

Attachment

Submitted filename: Dear.pdf

PONE-D-19-31278R1

Gene Polymorphisms of Patatin-like phospholipase domain containing 3 (PNPLA3), Adiponectin, Leptin in Diabetic Obese Patients

PLOS ONE

Dear Dr Hafez,

Thank you for submitting your manuscript to PLOS ONE. After careful consideration, we feel that it has merit but does not fully meet PLOS ONE’s publication criteria as it currently stands. Therefore, we invite you to submit a revised version of the manuscript that addresses the points raised during the review process.

In addition to the Academic Editor's comments from the previous decision, we have now obtained an assessment from an external reviewer. Please find their comments included below.

We would appreciate receiving your revised manuscript by May 10 2020 11:59PM. When you are ready to submit your revision, log on to https://www.editorialmanager.com/pone/ and select the 'Submissions Needing Revision' folder to locate your manuscript file.

If you would like to make changes to your financial disclosure, please include your updated statement in your cover letter.

To enhance the reproducibility of your results, we recommend that if applicable you deposit your laboratory protocols in protocols.io, where a protocol can be assigned its own identifier (DOI) such that it can be cited independently in the future. For instructions see: http://journals.plos.org/plosone/s/submission-guidelines#loc-laboratory-protocols

Please include the following items when submitting your revised manuscript:

• A rebuttal letter that responds to each point raised by the academic editor and reviewer(s). This letter should be uploaded as separate file and labeled 'Response to Reviewers'.
• A marked-up copy of your manuscript that highlights changes made to the original version. This file should be uploaded as separate file and labeled 'Revised Manuscript with Track Changes'.
• An unmarked version of your revised paper without tracked changes. This file should be uploaded as separate file and labeled 'Manuscript'.

Please note while forming your response, if your article is accepted, you may have the opportunity to make the peer review history publicly available. The record will include editor decision letters (with reviews) and your responses to reviewer comments. If eligible, we will contact you to opt in or out.

We look forward to receiving your revised manuscript.

Kind regards,

Artur Arikainen, Associate Editor, PLOS ONE

on behalf of

Nadia M Hamdy, Ph.D.

Academic Editor

PLOS ONE

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Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

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Reviewer #1: (No Response)

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2. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: (No Response)

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3. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: (No Response)

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Reviewer #1: (No Response)

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Reviewer #1: (No Response)

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6. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: 1. English editing is highly recommended throughout the manuscript.

2. Abstract:

- Rephrase line 35-37.

3. Introduction:

- Add more references on PNPLA3, lane 76

4. Methods:

- “Fresh venous blood samples” explain what do you mean by “fresh” this is not an accurate word here, explain?? And rephase.

- Add references for PNPLA3 amplification and genotyping sections.

- A figure for PNPLA3 results should be added.

5. Results:

- include a table with the demographic data of all participants.

- Rephrase lines from 190-194 to be more simple and comprehensive.

-

6. Discussion:

- Authors should compare results of genotypes distribution of the genes with other studies in Egypt, specially in controls.

- Authors should compare polymorphism results of all genes with those of other studies.

7. Limitations of the study:

- State clearly that the small sample numbers is a limitation in the study of polymorphism of these genes in the herein study.

8.

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Reviewer #1: No

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Attachments

Attachment

Submitted filename: REPLY.docx

Gene Polymorphisms of Patatin-like phospholipase domain containing 3 (PNPLA3), Adiponectin, Leptin in Diabetic Obese Patients

PONE-D-19-31278R2

Dear Dr. Hafez,

We are pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it complies with all outstanding technical requirements.

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With kind regards,

Nadia M Hamdy, Ph.D.

Academic Editor

PLOS ONE

Additional Editor Comments (optional):

Reviewers' comments: