Peer Review History
Original SubmissionSeptember 26, 2019 |
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PONE-D-19-27094 Aberrant HLA-DR expression in the conjunctival epithelium after autologous serum treatment in patients with graft-versus-host disease or Sjögren’s syndrome PLOS ONE Dear Assoc. Prof., Jirsova, Thank you for submitting your manuscript to PLOS ONE. After careful consideration, we feel that it has merit but does not fully meet PLOS ONE’s publication criteria as it currently stands. Therefore, we invite you to submit a revised version of the manuscript that addresses the points raised during the review process. The authors performed an observational study of subjects with dry eye either secondary to oGVHD or primary Sjogren's syndrome to correlate clinical symptoms with impression cytology of the conjunctiva and to determine the number of HLADR+ cells before and after treatment with autologous serum eye drops. The reviewers found some problems with the paper that the authors need to address. 1. It is noted that there are many confounders in study - sex, previous treatment, reliability of counting + HLADR cells from IC stained filters. These need to be evaluated. 2. It is not clear if the readers of IC were " blinded" to pre vs. post AS treatment when evaluating samples. We would appreciate receiving your revised manuscript by Feb 07 2020 11:59PM. When you are ready to submit your revision, log on to https://www.editorialmanager.com/pone/ and select the 'Submissions Needing Revision' folder to locate your manuscript file. If you would like to make changes to your financial disclosure, please include your updated statement in your cover letter. To enhance the reproducibility of your results, we recommend that if applicable you deposit your laboratory protocols in protocols.io, where a protocol can be assigned its own identifier (DOI) such that it can be cited independently in the future. For instructions see: http://journals.plos.org/plosone/s/submission-guidelines#loc-laboratory-protocols Please include the following items when submitting your revised manuscript:
Please note while forming your response, if your article is accepted, you may have the opportunity to make the peer review history publicly available. The record will include editor decision letters (with reviews) and your responses to reviewer comments. If eligible, we will contact you to opt in or out. We look forward to receiving your revised manuscript. Kind regards, Alexander V. Ljubimov, Ph.D. Academic Editor PLOS ONE Journal Requirements: 1. When submitting your revision, we need you to address these additional requirements. Please ensure that your manuscript meets PLOS ONE's style requirements, including those for file naming. The PLOS ONE style templates can be found at http://www.journals.plos.org/plosone/s/file?id=wjVg/PLOSOne_formatting_sample_main_body.pdf and http://www.journals.plos.org/plosone/s/file?id=ba62/PLOSOne_formatting_sample_title_authors_affiliations.pdf 2. Please provide additional details regarding participant consent. In the Methods section, please ensure that you have specified what type of consent you obtained (for instance, written or verbal) and whether the ethics committee approved this consent procedure. If verbal consent was obtained please state why it was not possible to obtain written consent and how verbal consent was recorded. If your study included minors, state whether you obtained consent from parents or guardians. 3. Please ensure that you have provided a limitations section in your manuscript. This section should discuss the absence of a control/placebo group in your study. We noticed you have some minor occurrence of overlapping text with the following previous work, which needs to be addressed: https://doi.org/10.3109/02713683.2013.824987 In your revision ensure you cite all your sources (including your own works), and quote or rephrase any duplicated text outside the methods section. Further consideration is dependent on these concerns being addressed. Additional Editor Comments: The authors performed an observational study of subjects with dry eye either secondary to oGVHD or primary Sjogren's syndrome to correlate clinical symptoms with impression cytology of the conjunctiva and to determine the number of HLADR+ cells before and after treatment with autologous serum eye drops. The reviewers found some problems with the paper that the authors need to address. 1. It is noted that there are many confounders in study - sex, previous treatment, reliability of counting + HLADR cells from IC stained filters. These need to be evaluated. 2. It is not clear if the readers of IC were " blinded" to pre vs. post AS treatment when evaluating samples. [Note: HTML markup is below. Please do not edit.] Reviewers' comments: Reviewer's Responses to Questions Comments to the Author 1. Is the manuscript technically sound, and do the data support the conclusions? The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented. Reviewer #1: Partly ********** 2. Has the statistical analysis been performed appropriately and rigorously? Reviewer #1: Yes ********** 3. Have the authors made all data underlying the findings in their manuscript fully available? The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified. Reviewer #1: Yes ********** 4. Is the manuscript presented in an intelligible fashion and written in standard English? PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here. Reviewer #1: Yes ********** 5. Review Comments to the Author Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters) Reviewer #1: Obervational study of subjects with dry eyes either secondary to oGVHD or primary Sjogren's syndrome to correlated clinical symptoms with impression cytology of the conjunctiva to determine number of HLADR + cells before and after treatment with autologous serum eye drops. Many confounders in study- sex , previous treatment, reliability of counting + HLAdr cells from IC stained filters. Also not clear if readers of IC were " blinded" to pre vs post AS treatment when evaluating samples. Study is interesting, but given potential confounders may be difficult to reach a conclusion. Most interesting part of study is the use of aberrant HLADR staining for positivity of conj. epithelial cells- this topic could be explored further ********** 6. PLOS authors have the option to publish the peer review history of their article (what does this mean?). 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Revision 1 |
Aberrant HLA-DR expression in the conjunctival epithelium after autologous serum treatment in patients with graft-versus-host disease or Sjögren’s syndrome PONE-D-19-27094R1 Dear Dr. Jirsova, We are pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it complies with all outstanding technical requirements. Within one week, you will receive an e-mail containing information on the amendments required prior to publication. When all required modifications have been addressed, you will receive a formal acceptance letter and your manuscript will proceed to our production department and be scheduled for publication. Shortly after the formal acceptance letter is sent, an invoice for payment will follow. To ensure an efficient production and billing process, please log into Editorial Manager at https://www.editorialmanager.com/pone/, click the "Update My Information" link at the top of the page, and update your user information. If you have any billing related questions, please contact our Author Billing department directly at authorbilling@plos.org. If your institution or institutions have a press office, please notify them about your upcoming paper to enable them to help maximize its impact. If they will be preparing press materials for this manuscript, you must inform our press team as soon as possible and no later than 48 hours after receiving the formal acceptance. Your manuscript will remain under strict press embargo until 2 pm Eastern Time on the date of publication. For more information, please contact onepress@plos.org. With kind regards, Alexander V. Ljubimov, Ph.D. Academic Editor PLOS ONE Additional Editor Comments (optional): The authors adequately replied to the critique. Reviewers' comments: |
Formally Accepted |
PONE-D-19-27094R1 Aberrant HLA-DR expression in the conjunctival epithelium after autologous serum treatment in patients with graft-versus-host disease or Sjögren’s syndrome Dear Dr. Jirsova: I am pleased to inform you that your manuscript has been deemed suitable for publication in PLOS ONE. Congratulations! Your manuscript is now with our production department. If your institution or institutions have a press office, please notify them about your upcoming paper at this point, to enable them to help maximize its impact. If they will be preparing press materials for this manuscript, please inform our press team within the next 48 hours. Your manuscript will remain under strict press embargo until 2 pm Eastern Time on the date of publication. For more information please contact onepress@plos.org. For any other questions or concerns, please email plosone@plos.org. Thank you for submitting your work to PLOS ONE. With kind regards, PLOS ONE Editorial Office Staff on behalf of Dr. Alexander V. Ljubimov Academic Editor PLOS ONE |
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