PONE-D-19-29232

Virus-associated anterior uveitis and secondary glaucoma:Diagnostics, clinical characteristics and surgical options

PLOS ONE

Dear Dr. Pohlmann,

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Kind regards,

Ahmed Awadein, MD, Ph.D, FRCS

Academic Editor

PLOS ONE

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 Dr. Dominika Pohlmann is participant in the BIH Charité Clinician Scientist Program funded by the Charité- Universitätsmedizin Berlin and the Berlin Institute of Health.

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Comments to the Author

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Reviewer #1: Yes

Reviewer #2: Yes

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2. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

Reviewer #2: I Don't Know

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Reviewer #1: Yes

Reviewer #2: Yes

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Reviewer #1: Yes

Reviewer #2: Yes

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5. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: I congratulate the authors for a well written and clear manuscript and a well designed study. The tables are very well presented although some table are rather exhaustive

The results are very informative and relevant to the clinical practice

Reviewer #2: The study question and idea: the idea is interesting and addressing an important gap in literature , the manuscript is also well written some points however still need to be addressed

1. the number of patients mentioned throughout the manuscript is 270 patients (=eyes), this is unclear as the authors mentioned in table (1) that 40 cases were bilateral

2. in table (1) : the authors mentioned that 123 case experienced an acute course of uveitis (hypertensive uveitis) , It is not understood why operation was needed during acute iridocyclitis which most cases can be temporarily controlled with medical treatment till remission occurs , even though in the rare circumstances where urgent surgery is needed , it is very difficult to judge whether the IOP has been surgically controlled or has it been the natural course of the disease . also evaluation of the success rate of surgical intervention in these cases is highly variable and can cause a statistical bias .

3. the surgical success rate was not clearly defined and the number of acute attacks could have been statistically correlated with the IOP control

4. it is not clear why the study is retrospective although the follow up plan is clearly defined in page 11 "...Patients

attended clinics preoperatively as well as follow-ups one day, six weeks, three months, six months, one

year, and two years postoperatively."

5. In table 4 : 2 cases needed CPC , this is needes further elaboartion as CPC in uveitic glaucoma is not favourable being associated with high incidence of pthisis bulbi so please outline the points of view in taking such decision

5. At which point was the "Goldmann-Witmer " test done ? during the attack of AAU or in CAU ? and how sensitive/ specific this test is

6. the conclusion "MIGS can be used as first-line-treatment in

individual cases, however, the device needs to be carefully chosen by experienced specialists based

on the individual needs of the patient. Filtrating glaucoma surgery remains the gold standard" can not be drawn as a fact from this study as it needs prospective evaluation and standardization of patients criteria and severity of glaucoma to be properly evaluated

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Reviewer #1: No

Reviewer #2: Yes: Riham S.H.M. Allam

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PONE-D-19-29232R1

Virus-associated anterior uveitis and secondary glaucoma:

Diagnostics, clinical characteristics, and surgical options

PLOS ONE

Dear Dr. Pohlmann,

Thank you for submitting your manuscript to PLOS ONE. After careful consideration, we feel that it has merit but does not fully meet PLOS ONE’s publication criteria as it currently stands. Therefore, we invite you to submit a revised version of the manuscript that addresses the points raised during the review process.

==============================

ACADEMIC EDITOR:

1- Please replace the word puncture (occasionally misspelled in some parts of the text as punction) with a more scholarly word like paracentesis or AC stab incision

2- Page 18 Place replace the word "positively tested" with "positive" or "positive results"

==============================

We would appreciate receiving your revised manuscript by Feb 21 2020 11:59PM. When you are ready to submit your revision, log on to https://www.editorialmanager.com/pone/ and select the 'Submissions Needing Revision' folder to locate your manuscript file.

If you would like to make changes to your financial disclosure, please include your updated statement in your cover letter.

To enhance the reproducibility of your results, we recommend that if applicable you deposit your laboratory protocols in protocols.io, where a protocol can be assigned its own identifier (DOI) such that it can be cited independently in the future. For instructions see: http://journals.plos.org/plosone/s/submission-guidelines#loc-laboratory-protocols

Please include the following items when submitting your revised manuscript:

• A rebuttal letter that responds to each point raised by the academic editor and reviewer(s). This letter should be uploaded as separate file and labeled 'Response to Reviewers'.
• A marked-up copy of your manuscript that highlights changes made to the original version. This file should be uploaded as separate file and labeled 'Revised Manuscript with Track Changes'.
• An unmarked version of your revised paper without tracked changes. This file should be uploaded as separate file and labeled 'Manuscript'.

Please note while forming your response, if your article is accepted, you may have the opportunity to make the peer review history publicly available. The record will include editor decision letters (with reviews) and your responses to reviewer comments. If eligible, we will contact you to opt in or out.

We look forward to receiving your revised manuscript.

Kind regards,

Ahmed Awadein, MD, Ph.D, FRCS

Academic Editor

PLOS ONE

[NOTE: If reviewer comments were submitted as an attachment file, they will be attached to this email and accessible via the submission site. Please log into your account, locate the manuscript record, and check for the action link "View Attachments". If this link does not appear, there are no attachment files to be viewed.]

While revising your submission, please upload your figure files to the Preflight Analysis and Conversion Engine (PACE) digital diagnostic tool, https://pacev2.apexcovantage.com/. PACE helps ensure that figures meet PLOS requirements. To use PACE, you must first register as a user. Registration is free. Then, login and navigate to the UPLOAD tab, where you will find detailed instructions on how to use the tool. If you encounter any issues or have any questions when using PACE, please email us at figures@plos.org. Please note that Supporting Information files do not need this step.

Virus-associated anterior uveitis and secondary glaucoma: Diagnostics, clinical characteristics, and surgical options

PONE-D-19-29232R2

Dear Dr. Pohlmann,

We are pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it complies with all outstanding technical requirements.

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With kind regards,

Ahmed Awadein, MD, Ph.D, FRCS

Academic Editor

PLOS ONE

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