Serum lipopolysaccharide neutralizing capacity in ischemic stroke

PONE-D-19-30948

Dear Dr. Pussinen,

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PLOS ONE

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1. We note that you have included the phrase “data not shown” in your manuscript. Unfortunately, this does not meet our data sharing requirements. PLOS does not permit references to inaccessible data. We require that authors provide all relevant data within the paper, Supporting Information files, or in an acceptable, public repository. Please add a citation to support this phrase or upload the data that corresponds with these findings to a stable repository (such as Figshare or Dryad) and provide and URLs, DOIs, or accession numbers that may be used to access these data. Or, if the data are not a core part of the research being presented in your study, we ask that you remove the phrase that refers to these data.

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Additional Editor Comments (optional):

The study is intriguing, well written and very promising. It is an important aspect for the clinical implications.

Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Yes

Reviewer #2: Yes

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2. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

Reviewer #2: Yes

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3. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #1: Yes

Reviewer #2: Yes

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4. Is the manuscript presented in an intelligible fashion and written in standard English?

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Reviewer #1: Yes

Reviewer #2: Yes

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5. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: This study inserts into a relevant, complex and constantly evolving topic of study, but it is focused on a very specific and poorly known aspect. The topic is covered in an accurate way. Work premises are highlighted, as well as the current state of knowledge and missing points; the experimental approach is well-conducted and results precisely analyzed, both from a statistical and interpretive point of view, leading to the highlighted conclusions. A well done research involving microbiological and clinical interesting implications.

Reviewer #2: The manuscript by Leskela and coworkers investigates the relationonship between LPS-activty and LPS-neutralizing capacity in ischemic stroke. Although the findings revealed no correlation, authors set up an interesting method to predict LPS-neutralizing capacity.

The manuscript is interesting and all procedures clearly described. The only weakness I found is that the manuscript would be not easily readable by not experts in the field.

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Reviewer #1: No

Reviewer #2: No