PONE-D-19-27091

Multigene phylogeny and taxonomy of Torula hydei and Dendryphion hydei spp. nov. from herbaceous litter in northern Thailand

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"The authors are grateful to the Mushroom Research Foundation, Chiang Mai, Thailand and

366 Key Research Program of Frontier Sciences of the Chinese Academy of Sciences (grant no.

367 QYZDY-SSW-SMC014) for supporting this research. We also acknowledge that Biology

368 Experimental Center, Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese

369 Academy of Sciences provide molecular laboratory facilities for molecular work. Rungtiwa

370 Phookamsak thanks CAS President’s International Fellowship Initiative (PIFI) for young staff

371 (grant no. Y9215811Q1) and National Science Foundation of China (NSFC) project code

372 31850410489 (grant no. Y81I982211) for financial support. Itthayakorn Promputtha grateful

to thank Chiang Mai University for partially support 373 of this research work. Rajesh Jeewon

374 would like to thank Mae Fah Luang University for giving him the opportunity as a visiting

375 professor to the Center of Excellence in Fungal Research and University of Mauritius for research

376 support. Peter E Mortimer would like to thank the National Science Foundation of China and

377 the South East Asian Biodiversity resources Institute, Chinese Academy of Sciences, for

378 financial support under the following grants: 41761144055, 41771063, Y4ZK111B01.

379 Mingkwan Doilom would like to thank the 5th batch of Postdoctoral Orientation Training

380 Personnel in Yunnan Province and the 64th batch of China Postdoctoral Science Foundation.

381 Jun-Fu Li thanks to Emeritus Prof. Kevin D. Hyde, Dr. Shaun Pennycook, Dr. Dhanushka

382 Wanasinghe, Hong-Bo Jiang, Dr. Zonglong Luo for their available suggestions and help."

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Comments to the Author

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Reviewer #1: Yes

Reviewer #2: Yes

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2. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

Reviewer #2: N/A

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Reviewer #1: Yes

Reviewer #2: Yes

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Reviewer #1: Yes

Reviewer #2: Yes

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5. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: Dear Junfu Li and co-authors,

Please see the attachment.

I have included several comments in the attached manuscript. Basically, this is a well written manuscript, however, i am not sure the content is strong enough for the Plos ONE.

Please remove the unnecessary paragraph from the introduction, and please improve your discussion. Most parts related to the Discussion should move to the notes part.

Please improve your phylogeny by including Rutola. Please refer "The Genera of Fungi – G5: Arthrinium, Ceratosphaeria, Dimerosporiopsis, Hormodochis, Lecanostictopsis, Lembosina, Neomelanconium, Phragmotrichum, Pseudomelanconium, Rutola, and Trullula"

Reviewer #2: Note: The following minor corrections may please be attended -

Page 8, Lines 26-27: Abstract: Delete the first sentence. Start the paragraph from second sentence – During our studies....

Pages 8-9, Lines: 44-48: Replace the sentences with this: Species in Torulaceae are known only by their asexual morphs which are characterized as follows:Superficial, effuse, greyish brown to black, powdery colonies; micro- or macronematous conidiophores, with or without apical branches; doliiform to ellipsoid or clavate, brown, smooth to verruculose, mono- to polyblastic conidiogenous cells which often remaining cupulate; subcylindrical, phragmosporous, acrogenous, brown, dry, smooth to verrucose conidia characteristically produced in branched chains

Page 9, L.50: accepted Dendryphion Wallr., besides Torula, within Torulaceae in Pleosporales.

Page 9, L.54: relationships of the taxa within the family ...

Page 11, L.94: northern Thailand. Among collected samples, Dendryphion hydei is recovered as another new .....

Page 11, L.96: updated phylogenetic tree with our new taxa for the family Torulaceae is provided in this ...

Page 12, L.113: Single conidium isolation ..

Page 24, L. 287: semi-macronematous, mononematous, ....

Page 24, L. 289: subglobose, arising from prostrate hyphae

Page 25, L. 315: al. [8] who re-classified Torula and ....

Page 25, L. 316: ......... Subsequent authors introduced new genera ....

Page 25, L. 320: Fungorum [23], but most of the described species lack DNA .....

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Reviewer #1: No

Reviewer #2: No

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Attachments

Attachment

Submitted filename: PONE-D-19-27091_reviewer.pdf

Multigene phylogeny and taxonomy of Dendryphion hydei and Torula hydei spp. nov. from herbaceous litter in northern Thailand

PONE-D-19-27091R1

Dear Dr. PROMPUTTHA,

We are pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it complies with all outstanding technical requirements.

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Tzen-Yuh Chiang

Academic Editor

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Additional Editor Comments (optional):

Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. If the authors have adequately addressed your comments raised in a previous round of review and you feel that this manuscript is now acceptable for publication, you may indicate that here to bypass the “Comments to the Author” section, enter your conflict of interest statement in the “Confidential to Editor” section, and submit your "Accept" recommendation.

Reviewer #1: All comments have been addressed

Reviewer #2: All comments have been addressed

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2. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Yes

Reviewer #2: Yes

**********

3. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

Reviewer #2: Yes

**********

4. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #1: Yes

Reviewer #2: Yes

**********

5. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.

Reviewer #1: Yes

Reviewer #2: Yes

**********

6. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: Dear Author

Thanks for addressing the comments raised during previous round.

I am happy with the MS and now it ready for publish

Reviewer #2: The authors have incorporated all corrections and suggestions made by the reviewers' and the manuscript is in very good shape. The paper is now recommended for publication.

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Reviewer #1: No

Reviewer #2: No