PONE-D-19-31968

Upregulation of Long Non-Coding RNA ROR1-AS1 Promotes Cell Growth and Migration in Bladder Cancer by Regulation of MiR-504

PLOS ONE

Dear Mr Chen,

Thank you for submitting your manuscript to PLOS ONE. After careful consideration, we feel that it has merit but does not fully meet PLOS ONE’s publication criteria as it currently stands. Therefore, we invite you to submit a revised version of the manuscript that addresses the points raised during the review process.

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Wen-Jun Tu

Academic Editor

PLOS ONE

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2. We noticed you have some minor occurrence(s) of overlapping text with the following previous publication(s), which needs to be addressed:

https://doi.org/10.1016/j.canlet.2016.01.051

https://doi.org/10.18632/oncotarget.17956

In your revision ensure you cite all your sources (including your own works), and quote or rephrase any duplicated text outside the Methods section. Further consideration is dependent on these concerns being addressed.

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Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Yes

Reviewer #2: Yes

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2. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

Reviewer #2: Yes

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3. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #1: Yes

Reviewer #2: Yes

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4. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.

Reviewer #1: Yes

Reviewer #2: Yes

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5. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: Whether the RT-qPCR experiment was repeated 3 times? the author needs to indicate in the method.

2.The sequences of shRNA-ROR1-AS1, shRNA-NC, miR-504 inhibitor and inhibitor NC should be added in the method.

3.The reason for choosing T24 and 5637 for functional assays, why not used J82, 253J and RT4

4.The scale bar should added in the images of wound scratch assay.

5.In discussion, they should discuss the role of ROR1-AS1 in other cancers and summarize them.

6.LncRNAs is very basic and information about the classification criteria is lacking. Therefore, it is suggested to mention and to add the corresponding references.

7.Please specifiy de size range of tissues used for RNA extraction.

8.There are some grammatical and wording errors in the paper. It is recommended that professionals revise it.

Reviewer #2: Increasing evidences showed that multiple long non-coding RNAs (lncRNAs) act crucial regulatory functions in the pathogenesis of bladder cancer. This study aimed to determine the expression and clinical significance of ROR1 antisense RNA (ROR1-AS1) from patients with bladder cancer, and to identify the potential role and mechanism underlying ROR1-AS1-related cancer progression. The results showed that increased ROR1-AS1 promotes cell growth and migration of bladder cancer via regulation of miR-504, and ROR1-AS1 may be used as a prognostic biomarker and therapeutic target for bladder cancer.

This study is interesting and clinically relevant, however there are a number of grammatical errors and issues regarding data collection that should be addressed.

Comments

1. The grammar needs to be edited throughout.

2. The introduction section, the aim of this study and more detailed research background should be presented.

3. “This research was approved by the Ethics Committee of the First Affiliated Hospital of Nanchang University’ the approval no should be present.

4. How many patients and bladder tissues were collected?

5. Human bladder tissue and tumor-adjacent normal bladder tissues were matched? Matched what?

6. Discussion: should be focused on the own results, It is written general and seems to be vague.

7. Kaplan-Meier survival analysis was used in the results section, please showed it in the method section.

8. Figure 2. Comments and headings should be in the same direction.

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Reviewer #1: No

Reviewer #2: No

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Upregulation of Long Non-Coding RNA ROR1-AS1 Promotes Cell Growth and Migration in Bladder Cancer by Regulation of MiR-504

PONE-D-19-31968R1

Dear Dr. Chen,

We are pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it complies with all outstanding technical requirements.

Within one week, you will receive an e-mail containing information on the amendments required prior to publication. When all required modifications have been addressed, you will receive a formal acceptance letter and your manuscript will proceed to our production department and be scheduled for publication.

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With kind regards,

Wen-Jun Tu

Academic Editor

PLOS ONE

Additional Editor Comments (optional):

Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. If the authors have adequately addressed your comments raised in a previous round of review and you feel that this manuscript is now acceptable for publication, you may indicate that here to bypass the “Comments to the Author” section, enter your conflict of interest statement in the “Confidential to Editor” section, and submit your "Accept" recommendation.

Reviewer #1: All comments have been addressed

Reviewer #2: All comments have been addressed

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2. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Yes

Reviewer #2: Yes

**********

3. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

Reviewer #2: Yes

**********

4. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #1: Yes

Reviewer #2: Yes

**********

5. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.

Reviewer #1: Yes

Reviewer #2: Yes

**********

6. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: The problems raised in a previous round were reviewed and corrected by the authors carefully. And the English language has be revised and corrected . As a result, I think the current revised manuscript is suitable for publication.

Reviewer #2: The Authors had thoroughly revised the manuscript and addressed requested changes and improvements。 No further comments. Thanks

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7. PLOS authors have the option to publish the peer review history of their article (what does this mean?). If published, this will include your full peer review and any attached files.

If you choose “no”, your identity will remain anonymous but your review may still be made public.

Do you want your identity to be public for this peer review? For information about this choice, including consent withdrawal, please see our Privacy Policy.

Reviewer #1: No

Reviewer #2: No