PONE-D-19-18869

Short-term treatment with a peroxisome proliferator-activated receptor α agonist influences plasma one-carbon metabolites and B-vitamin status in rats

PLOS ONE

Dear Mr. Lysne,

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The reviewer finds your study to be of interest; however they raise a number of issues which you should consider in revising your manuscript. In particular, please respond to the comment regarding hepatic PPARγ activation in your previous publication (ref 36) as it relates to the current study. The reviewer also requests that you perform some additional analyses to avoid duplication and build upon results presented in your previous publication.

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Academic Editor

PLOS ONE

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Reviewer #1: Partly

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Reviewer #1: N/A

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Reviewer #1: Yes

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Reviewer #1: Yes

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5. Review Comments to the Author

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Reviewer #1: The results showed in the manuscript give some significent explain of the effect of PPARα and PPARγ on one-carbon matbolism. However, the paper is unsuitable for publication in its present form. There are some problem and suggestion as followed.

1 Energy intake should be describle more detail.

2 Although the PPARα and PPARγ mRNA expression were anaylazied ( in reference 36 ), there no difference of PPARγ mRNA level between PPARγ and control, if PPARγ was not activated in haptic and adipose tissue, how do you get the conclusion that PPARγ activatoin has rarely accosiated with one carbon matebolism? In fact, according the experiment data showed in reference 36. It seemed that PPARα mRNA expression significantly increased compared with control only in heptaic tissue, howerer, it did not seem the PPARα agonist had significant effect on PPARα expression in adipose tissue.

3 It is not approved to separate the data of PPAR expression from the data of one-carbon matebolism products. Please provide data of wester-blotting or ELISA to certify PPARα and PPARγ expression at higher level under the intervention of agonist in current study, avoiding repetition data in literature 36.

4 The describe of PPARα expression should be showed in discussion in line 91-94.

5 The analysis of genes expresssion of serine-hydroxymethyltransferase, ACMS dehydrogenase , QAPRT, methylmalonyl-CoA mutase are necessary, otherwise, the avalible evidence is not very convincing.

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Reviewer #1: No

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Short-term treatment with a peroxisome proliferator-activated receptor α agonist influences plasma one-carbon metabolites and B-vitamin status in rats

PONE-D-19-18869R1

Dear Dr. Lysne,

We are pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it complies with all outstanding technical requirements.

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With kind regards,

Nick Ashton, PhD

Academic Editor

PLOS ONE

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