PONE-D-19-16566

Assessment of energy expenditure during high intensity cycling and running using a heart rate and activity monitor in young active adults

PLOS ONE

Dear Dr Klass,

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Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Partly

Reviewer #2: Yes

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2. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

Reviewer #2: Yes

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3. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #1: Yes

Reviewer #2: Yes

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4. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.

Reviewer #1: Yes

Reviewer #2: Yes

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5. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: Brief Summary:

Authors aimed to evaluate the accuracy of the Actiheart to assess activity related energy expenditure on HR and activity counts in young and very active subjects during running and cycling. To achieve this aim, they estimated total energy expenditure by using Weir equation and then compare it with data obtained from Activeheart based on different. However some methodological issues should be addressed. I would advise “major revision” for this manuscript based on general comments, as describe below:

General comments:

Abstract: the method for indirect calorimetry is not mentioned. In my opinion, it’s very important as this method is used as reference to verify the accuracy of the Actiheart data.

HR sleeping: I cannot understand the physiological relevance for the present study. Probably, its relevance is missing.

Could explain please why 2 individuals were excluded ? How data is lost? And the reliability of the data obtained from Actiheart ?

The main question of the present study is that you missed the determinants of energy expenditure that includes, body size, body composition, sex (as you used both, 4 female and 16male), age, physical fitness, hormonal status. How to ensure that there was no influence of these factors? Is any way to consider these factors in your data analysis ? Please, include a sentence in the introduction and discussion with information explaining how these determinants can influence your data ?

Reviewer #2: Abstract

Please add gender and age of the sample

Introduction

Would be better to add hypothesis

Methods

Participants

- Please add exclusion criteria

- Please explain how the activity level of the sample was determined

Protocol

- Please mention the time of the day of the experimental sessions

Sleeping HR

- Please add reference citation(s) to the sentence starting with “To that aim subjects had to …”

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Reviewer #1: Yes: Sílvia Rocha-Rodrigues

Reviewer #2: No

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Assessment of energy expenditure during high intensity cycling and running using a heart rate and activity monitor in young active adults

PONE-D-19-16566R1

Dear Dr. Klass,

We are pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it complies with all outstanding technical requirements.

Within one week, you will receive an e-mail containing information on the amendments required prior to publication. When all required modifications have been addressed, you will receive a formal acceptance letter and your manuscript will proceed to our production department and be scheduled for publication.

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With kind regards,

Filipe Manuel Clemente, PhD

Academic Editor

PLOS ONE

Additional Editor Comments (optional):

Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. If the authors have adequately addressed your comments raised in a previous round of review and you feel that this manuscript is now acceptable for publication, you may indicate that here to bypass the “Comments to the Author” section, enter your conflict of interest statement in the “Confidential to Editor” section, and submit your "Accept" recommendation.

Reviewer #1: All comments have been addressed

Reviewer #2: All comments have been addressed

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2. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Yes

Reviewer #2: Yes

**********

3. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

Reviewer #2: Yes

**********

4. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #1: Yes

Reviewer #2: Yes

**********

5. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.

Reviewer #1: Yes

Reviewer #2: Yes

**********

6. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: The authors exceeded all the questions and suggestions of reviewers. Based on that, I recommend accept for this publication.

Reviewer #2: (No Response)

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7. PLOS authors have the option to publish the peer review history of their article (what does this mean?). If published, this will include your full peer review and any attached files.

If you choose “no”, your identity will remain anonymous but your review may still be made public.

Do you want your identity to be public for this peer review? For information about this choice, including consent withdrawal, please see our Privacy Policy.

Reviewer #1: Yes: Sílvia Rocha-Rodrigues

Reviewer #2: No