PONE-D-19-15939

Metabolomic response of Euglena gracilis and its bleached mutant strain to light

PLOS ONE

Dear Dr. Wang,

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Haitao Shi

Academic Editor

PLOS ONE

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1. Thank you for including the following funding information within the acknowledgements section of your manuscript; "This work was supported by National Natural Science Foundation of China (31670116), the Guangdong Innovation Research Team Fund (2014ZT05S078), and the Shenzhen Grant Plan for Science & Technology (JCYJ20160308095910917, JCYJ20170818100339597), used for the design of the study, data collection, data analysis and manuscript writing. The authors gratefully acknowledge the supports from the Instrumental Analysis Center of Shenzhen University (Xili Campus)."

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Reviewer #1: Yes

Reviewer #2: Partly

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Reviewer #1: Yes

Reviewer #2: Yes

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Reviewer #1: Yes

Reviewer #2: No

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Reviewer #1: Yes

Reviewer #2: No

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Reviewer #1: This is an interesting study of a unique type of algae. The experimental design is sound and the results are useful. There are a few points needs to be amended or addressed.

The last paragraph of the introduction, no result should be presented or discussed in the introduction.

Line 103, what does it mean "The solvent was delayed by 10 min"?

Line 191, why the low growth of growth of the OflB2 mutant is unexpected? this needs better explanation.

It is hard for readers to understand when conclusion is made from unpublished data. Suggest to delete or modify the 2nd paragraph of the summary.

Reviewer #2: The manuscript applied gas chromatography-mass spectrometry (GC-MS) to determine the metabolomes of wild-type (WT) Euglena gracilis and its bleached mutant OflB2 under light stimulation. These metabolomic changes after light stimulation are of great significance to the development of Euglena chloroplasts and their communications with the nucleus. However, The manuscript is relatively simple, the authors should consider the following points:

1. Figure 1 cannot be found.

2. Morphological changes of chlorophyll and other physiological indicators should be observed

3. Add GC-MS Analysis of Raw Data Graph.

3. It is suggested that genomics, transcriptome and proteomics data be published together to better reveal the mechanism of light effects on them.

4.There are some grammatical errors in the manuscript,please modify the language further.

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Reviewer #1: No

Reviewer #2: No

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Metabolomic re​sponse of Euglena gracilis and its bleached mutant strain to light

PONE-D-19-15939R1

Dear Dr. Wang,

We are pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it complies with all outstanding technical requirements.

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With kind regards,

Haitao Shi

Academic Editor

PLOS ONE

Additional Editor Comments (optional):

Reviewers' comments: