PONE-D-19-16374

Organic resolution function and effects of non-colloidal platinum nanoparticles on bacteria and organic matter

PLOS ONE

Dear Professor Kazuhiro,

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Amitava Mukherjee, ME, Ph.D.

Academic Editor

PLOS ONE

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Reviewer #1: Yes

Reviewer #2: No

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2. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

Reviewer #2: Yes

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Reviewer #1: Yes

Reviewer #2: No

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Reviewer #1: Yes

Reviewer #2: No

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5. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: The submitted paper entitled" Organic resolution function and effects of non colloidal platinum nanoparticles on bacteria and organic matter" shows the antibacterial effects,its potential to decompose LPS and its use in peridontal applications. The manuscript is well written and deserves a publication with some minor revisions. Below is the some comments and suggestions to improve the quality of manuscript.

Technical comments

1. In material method section (Bacterial culture and regulation),write down the parameters for shaking at what rpm shaking was kept for aerobic bacteria

2. Add a section of stock solution preparation of NPs under material method

3. In material and method section "antibacterial testing" add a reference for the protocol utilized

4. In the discussion section, line no. 243,explain the mechanism of interaction of aerobic and anaerobic bacteria with NPs

5. In the discussion section, line no. 268, when LPS reacts with NPs is there any charge based interaction in Gram negative bacteria

6. Figure number 3 add an X axis demarcation

Reviewer #2: Authors reported non-colloidal platinum nanoparticles functional potential for decomposing organic matter. As the author pointed out, the NC-PtNPs increase fragmentation and decomposition of albumin, which may limit their practical applications because besides damging the LPs, the proposed NPs can be toxic to host through damaging the albumin. However, I found several serious problems in this manuscript and cannot accept this manuscript in a present form.

1. The authors claim non-colloidal form, but the particle size is 2–19 nm and suspended in water. Comment on it.

2. Although the NPs are obtained from commercial source, its size should be verified after suspended in water and data should be presented.

3. What will happen to fate of the NPs size and morphology after contacting either albumin or LPS.

4. What is mechanism of decomposition?

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Reviewer #1: Yes: Dr. Ankita Mathur

Reviewer #2: No

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Attachments

Attachment

Submitted filename: PONE-D-19-16374_reviewer comments 1.pdf

[EXSCINDED]

Organic resolution function and effects of platinum nanoparticles on bacteria and organic matter

PONE-D-19-16374R1

Dear Dr. Kazuhiro,

We are pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it complies with all outstanding technical requirements.

Within one week, you will receive an e-mail containing information on the amendments required prior to publication. When all required modifications have been addressed, you will receive a formal acceptance letter and your manuscript will proceed to our production department and be scheduled for publication.

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With kind regards,

Amitava Mukherjee, ME, Ph.D.

Academic Editor

PLOS ONE

Additional Editor Comments (optional):

Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. If the authors have adequately addressed your comments raised in a previous round of review and you feel that this manuscript is now acceptable for publication, you may indicate that here to bypass the “Comments to the Author” section, enter your conflict of interest statement in the “Confidential to Editor” section, and submit your "Accept" recommendation.

Reviewer #1: All comments have been addressed

Reviewer #2: All comments have been addressed

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2. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Yes

Reviewer #2: Yes

**********

3. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

Reviewer #2: Yes

**********

4. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #1: Yes

Reviewer #2: Yes

**********

5. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.

Reviewer #1: Yes

Reviewer #2: Yes

**********

6. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: All the questions that were raised while reviewing had been addressed and thereby paper could be published.

Reviewer #2: The authors considerably improved the manuscript titled: Organic resolution function and effects of platinum nanoparticles on bacteria and organic matter. It may be acceptable

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7. PLOS authors have the option to publish the peer review history of their article (what does this mean?). If published, this will include your full peer review and any attached files.

If you choose “no”, your identity will remain anonymous but your review may still be made public.

Do you want your identity to be public for this peer review? For information about this choice, including consent withdrawal, please see our Privacy Policy.

Reviewer #1: No

Reviewer #2: Yes: Anbazhagan Veerappan