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Fig 1.

Distribution of Weirs in Zhejiang Province, China.

(a) Provincial-scale map; (b) Majinxi River Basin (the sampling site for experimental fish); (c)Yong’anxi River Basin. Base Map Source Attribution: The base maps in this figure were generated using elevation data from the ASTER Global Digital Elevation Model V3 (ASTER GDEM v3; NASA/METI, https://doi.org/10.5067/ASTER/ASTGTM.003) and the NASA Shuttle Radar Topography Mission Global 1 arc second V3 (SRTMGL1 v3; NASA/JPL, https://doi.org/10.5067/MEaSUREs/SRTM/SRTMGL1.003). These datasets are publicly available through the NASA Earthdata platform (https://earthdata.nasa.gov/). Data gaps in the ASTER GDEM were filled using SRTMGL1 v3 data.

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Fig 2.

Configuration of the experimental setup (Unit: cm).

(a) Plan view; (b) Section view.

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Table 1.

Passage success rate of A. fasciatus at successive weir stages.

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Table 1 Expand

Fig 3.

Passage success rate (PSR) of A. fasciatus at three successive weir stages across four body length classes.

Bars represent the mean ± standard deviation (SD) of the two biological replicate trials within each body length class. All inferential statistical analyses in this study are based on the 8 independent trial group values (4 classes × 2 replicates).

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Fig 4.

Photographs of A. fasciatus during upstream passage attempts.

(a) PLS; (b) LSS. Leaping individuals are marked with red circles.

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Table 2.

Proportion of passage strategy usage of A. fasciatus at the first weir, by body length class..

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Fig 5.

Relationship between mean body length per trial group and the proportion of PLS/LSS usage.

Each data point represents one of the 8 independent trial groups. All linear regression analyses are based on these 8 independent data points..

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Fig 6.

Behavioral parameter analysis of upstream migration in A. fasciatus of different body lengths.

(a) (b) Relationship between body length and takeoff position; (c) (d) Relationship between body length and leap distance; (e) (f) Relationship between body length and leap height. Each data point represents one of the 8 independent trial groups, showing the mean body length and median kinematic value for that group. All linear regression analyses (trend lines) are based on these 8 independent data points, with R² and p values reported in the text.

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Table 3.

Statistics of swimming (Sprint) distance within the leaping-swimming strategy for A. fasciatus of different body lengths.

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Fig 7.

Schematic of weir crest profile and pool layout.

(a) hydraulic head drop; (b) pool depth; (c) inter-weir pool length).

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