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Table 1.

PICOS Framework for the Systematic Review and Meta-Analysis on Epitope-Based and Peptide-Based Vaccines Against Avian Pathogenic Escherichia coli (APEC) in Poultry.

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Fig 1.

PRISMA flow diagram for study selection of APEC vaccine studies in poultry.

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Table 2.

Summary of included studies evaluating the efficacy of epitope-based, peptide-based, and alternative vaccine strategies.

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Fig 2.

Traffic light and summary plots of Cochrane ROB 2.0 assessment for randomized controlled trials.

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Fig 3.

Traffic light and summary plots of ROBINS-I assessment for non-randomized studies.

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Fig 4.

Forest plot of the effect of epitope-based, peptide-based, and alternative vaccine strategies on morbidity prevention in poultry challenged with APEC.

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Fig 5.

Funnel plot assessing publication bias for morbidity prevention outcomes.

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Fig 6.

Forest plot of the effect of epitope-based, peptide-based, and alternative vaccine strategies on mortality prevention in poultry infected with APEC.

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Fig 7.

Funnel plot assessing publication bias for mortality outcomes.

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Table 3.

Summary of secondary outcomes from included studies evaluating the impact of epitope-based, peptide-based, and alternative vaccine strategies against APEC in poultry.

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Table 4.

Subgroup analysis of vaccine efficacy against APEC in poultry (n = 10).

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Table 5.

Summary of findings on the role of ML in epitope prediction and vaccine optimization for APEC vaccines.

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Fig 8.

Conceptual framework for integrating artificial intelligence and machine learning into poultry vaccine development.

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