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Table 1.

Demographic and clinical characteristics of breast cancer patients and healthy controls.

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Table 2.

Serum immune checkpoint levels in BC patients and healthy controls.

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Fig 1.

Serum immune checkpoint levels in BC patients versus healthy controls.

Bar graphs display the mean concentrations (± standard error) of CD80, CTLA-4, IDO, CD86, GITRL, HVEM, ICOS, PD-1, PD-L1, LAG-3, TIM-3, and TLR-2 IC proteins. Statistically significant differences between groups are indicated as * p < .05, **p < .005, ***p < .0001.

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Fig 2.

Differential expression of IC proteins in TNBC versus non-TNBC patients.

Mean serum concentrations (± standard error) of CD28, HVEM, CD40, LAG-3, PD-1, TIM-3, CTLA-4, and TLR-2 IC proteins in TNBC (TN(+), pink bars) and non-TNBC (TN(–), blue bars) patients. Significant differences in expression levels between groups are indicated by red asterisks (*).

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Fig 3.

Spearman rank correlation heatmap of serum IC proteins in breast cancer patients.

Heatmap illustrates pairwise Spearman correlation coefficients among BTLA, CD27, CD28, CD40, CD80, CD86, CTLA-4, GITR, GITRL, HVEM, ICOS, LAG-3, PD-1, PD-L1, TIM-3, and TLR-2 IC proteins. Color intensity reflects correlation strength and direction, with blue indicating positive correlations and red indicating negative correlations.

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Fig 3 Expand

Table 3.

Serum immune checkpoint profiles associated with TNBC and clinical outcomes.

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Table 3 Expand