Table 1.
Primers used for specifically recognizing the tlh gene of V. parahaemolyticus by LAMP.
Table 2.
Collection sites of aquaculture water samples.
Fig 1.
Extracted genomic DNA in water (a), crude DNA in water (b), extracted genomic DNA in TS1 through TS8 (c), and crude DNA in TS1 through TS8 (d), respectively, served as templates for LAMP to detect the tlh gene of V. parahaemolyticus. Resulting amplicons were analyzed by gel electrophoresis and the fluorescent DNA dye GeneFinder™. Panel (a): Lane 1: negative control; Lanes 2–8: extracted genomic DNA served as template was extracted from 106, 105, 104, 103, 102, 101, and 100 CFU/µL bacterial cells, respectively. Panel (b): Lane 1: negative control; Lanes 2–8: boiled bacterial suspensions of 106, 105, 104, 103, 102, 101, and 100 CFU/µL, respectively, served as DNA template. PCR tubes aligned above each gel electrophoresis lane correspond to visualization results obtained upon addition of GeneFinder™.
Table 3.
Physicochemical parameters (mean value, n = 3) of treated aquaculture water samples.
Fig 2.
Pearson correlation matrix between all pairwise combinations of properties, as well as between failed LAMP reactions and individual variables.
Within the inset, values denote mean salinity levels (n = 3), with error bars representing the SD of these means. Significant differences (P < 0.05) are indicated by distinct superscript letters above bars.
Fig 3.
Extracted genomic DNA (a) and crude DNA (b) in TS2 and modified TS2 served as DNA templates for LAMP assays to detect the tlh gene of V. parahaemolyticus.
Lane 1: negative control; Lanes 2–8: salinity of 7.61‰, 10.00‰, 13.00‰, 16.00‰, 19.00‰, 21.00‰, and 24.00‰, respectively. PCR tubes aligned above each gel electrophoresis lane correspond to visualization results obtained upon addition of GeneFinder™.
Table 4.
Visualization results of dLAMP assays using 10 µL boiled TS2 and salinity-modified TS2 containing various amount of V. parahaemolyticus to serve as DNA templates (n = 3).
Fig 4.
Specificity verification of the LAMP assay challenged with three V. parahaemolyticus strains, eight other Vibrio spp. strains, and eight non-Vibrio strains.
Fig 5.
Procedure and feature of the rapid detection of V. parahaemolyticus using boiled aquaculture water as DNA templates for LAMP.