Table 1.
Summary of eDNA results from trial A, comparing samples from sea net pens and treatment chambers during thermal delousing at 16 Norwegian Atlantic salmon farms with known Pasteurellosis status.
Fig 1.
Samples storage trial results.
Line plot of Cq values (inverted y-axis) from Ph. atlanticus subsp. atlanticus qPCR analysis from spiked sea water samples with low and high bacterial load, stored either at 4 °C or 22 °C for up to nine days.
Fig 2.
A bar plot showing proportion of detections from Ph. atlanticus subsp. atlanticus qPCR analysis of eDNA samples from field trial A where samples were collected from Norwegian Atlantic salmon farms with known pasteurellosis status. Individual points (circles) represent raw observations (0 = not detected, 1 = detected), jittered horizontally for visibility. Water samples were collected from pen and treatment chamber (before, after 1/3, after 2/3 and after full thermal treatment). Only results from farms where one or more samples were PCR positive for Ph. atlanticus subsp. atlanticus are included. Detections from filters and pellet are included.
Table 2.
Summary of eDNA results from field trial B. Screening of samples from Norwegian Atlantic salmon farms presumed non-infected with Ph. atlanticus. The clinical state post eDNA analysis was evaluated and reported by individual farm fish health personnel.
Fig 3.
A bar plot showing proportion of detections from Ph. atlanticus subsp. atlanticus qPCR analysis of eDNA samples from field trial B where samples were collected from Norwegian Atlantic salmon farms with no previous history of Pasteurellosis. Individual points (circles) represent raw observations (0 = not detected, 1 = detected), jittered horizontally for visibility. Water samples were collected from the treatment chamber before and after thermal treatment. Only results from farms where one or more samples were PCR positive for Ph. atlanticus subsp. atlanticus were included.
Fig 4.
A bar plot showing proportion of detections of Ph. atlanticus subsp. atlanticus qPCR analysis from eDNA samples from field trial C. Individual points (circles) represent raw observations (0 = not detected, 1 = detected), jittered horizontally for visibility. Samples were collected from the anaesthetic tank from two Norwegian Atlantic salmon farms with diagnosed Ph. atlanticus infection, before and after lice treatment. Only results from farm C1 were included in figure as all samples from farm C2 were scored negative for Ph. atlanticus. Due to contamination of samples during the automated DNA extraction (Qiacube) samples with Cq value ≤ 33 were scored as PCR positive, while samples with a Cq value > 33 were scored as negative.