Fig 1.
(A) Diagrams of the Traits of LX and LC Medicinal Materials; (B) Determination Results of Trait Indicators of LX and LC(* p < 0.05).
Fig 2.
(A) LX and LC color analysis chart; (B) PCA Score Plot of Color Difference between LX and LC.
Fig 3.
(A) LC and LX sensor response radar chart; (B) PCA of LC and LX electronic nose data.
Fig 4.
(4: chlorogenic acid, 5: tetramethylpyrazine, 7: ferulic acid, 9: senkyunolide I, 10: senkyunolide H, 16: conifery ferulate, 17: senkyunolide A, 18: 3-n-butylphthalide, 20: ligustilide, 21: 3-butenylphthalide); (B) LC and LX HPLC fingerprint.
Fig 5.
LC and LX cluster analysis tree diagram.
Table 1.
Eigenvalue and variance contribution rate.
Fig 6.
Orthogonal partial least squares discriminant analysis (OPLS-DA) analysis of LC and LX.
(A) OPLS-DA score and load map; (B) Results of 200 cross-validations of OPLS-DA model; (C) The OPLS-DA model obtained a histogram with VIP value > 1.0, and a confidence level of 95%; (D) Box diagram for the determination of chemical composition content. The significant difference is determined by p < 0.05 (*), 0.01 (**), 0.001 (***).
Fig 7.
(A) The types of volatile components in LC and LX; (B) OPLS-DA score graph of LC and LX based on HS-SPME-GC-MS; (C) Permutation test results; (D) VIP value > 1, p < 0.05 difference component histogram; (E) The content of 11 different components.
(Cyclohexane, 1-methylene-4-(1-methylethenyl)-: C1; (E)-3-Butylidene-4, 5-dihydroisobenzofuran-1(3H)-one: E3; Bicyclo[3.1.1]heptane, 6, 6-dimethyl-2-methylene-(1S)-: B3; 5-Pentylcyclohexa-1, 3-diene: 5P; Bicyclo[3.1.0]hexane, 4-methylene-1-(1-methylethyl)-: Bh; Hexadecanoicacid, methylester: He; Phenol, 5-ethenyl-2-methoxy-: Ph; (1R)-2, 6, 6-Trimethylbicyclo[3.1.1]hept-2-ene: 1R; 12, 15-Octadecadienoic acid, methyl ester: Oc; 2-Methoxy-4-vinylphenol: 2M; 2-Octanol: 2O.) The significant difference is determined by p < 0.05 (*), 0.01 (**), 0.001 (***).
Fig 8.
The appearance, odor and differential chemical correlation heat map of LC and LX.
(Cyclohexane, 1-methylene-4-(1-methylethenyl)-: C1; (E)-3-Butylidene-4, 5-dihydroisobenzofuran-1(3H)-one: E3; Bicyclo[3.1.1]heptane, 6, 6-dimethyl-2-methylene-(1S)-: B3; 5-Pentylcyclohexa-1, 3-diene: 5P; Bicyclo[3.1.0]hexane, 4-methylene-1-(1-methylethyl)-: Bh; Hexadecanoicacid, methylester: He; Phenol, 5-ethenyl-2-methoxy-: Ph; (1R)-2, 6, 6-Trimethylbicyclo[3.1.1]hept-2-ene: 1R; 12, 15-Octadecadienoic acid, methyl ester: Oc; 2-Methoxy-4-vinylphenol: 2M; 2-Octanol: 2O). The significant difference is determined by p < 0.05 (*), 0.01 (**), 0.001 (***).