Fig 1.
Bayesian phylogram constructed from concatenated cox1, rbcL and RuBisCo sequences of Eucheumatopsis samples.
Support values are shown only for the main branches and are expressed as Bayesian posterior probabilities.
Fig 2.
Morphotype 1 (M1), 2 (M2) and 3 (M3) of E. isiformis from Yucatan and their cystocarp diversity.
(A) M1; (B) M2; (C) M3. Scale bar: complete specimens = 10 cm; cystocarps = 5 mm.
Table 1.
Quantitative data on the reproductive characters of Eucheumatopsis isiformis from Dzilam de Bravo, Yucatan.
Fig 3.
Mature cystocarps collected in March 2023.
Panels A, C, and E correspond to a cystocarp with diameter of 1.65 mm; whereas panels B, D, and F correspond to a cystocarp with diameter of 1.76 mm. Labels: o = ostiole, f = central fusion cell, p = pericarp: g = gonimoblast packs, cg = carposporangia, ca = carpospores and b = union base of gonimoblast packs arranged in candelabra-like structure.
Fig 4.
Variation in cystocarp diameter among morphotypes (M1, M2 and M3) observed from March 2022 to March 2023.
Boxplots represent, from bottom to top, the first quartile, median, third quartile and maximum values, including any outliers. **indicates a statistically significant difference (p < 0.001).
Fig 5.
Morphology of specimens in the tetrasporophyte phase of E. isiformis collected in June and August 2024.
Scale bar of sections = 5 mm and complete specimens = 10 cm.
Fig 6.
Tetrasporangia of E. isiformis observed in June and August 2024 collections.
Scale bar = 20 µm.
Fig 7.
Carpospores obtained under treatment with 10-3 M putrescine (PUT) in March 2024 after 10 days of acclimatization.
(A) dark after 36-37 h of induction. (B) 20 µmol·m-2·s-1 after 38 h of induction. (C) 40 µmol·m-2·s-1 after 34-38 h of induction. Scale bar = 20 µm.
Fig 8.
Number of carpospores obtained in March 2024 after 10 days of acclimatization.
Irradiances levels are indicated as 0, 20 and 40 µmol·m-2·s-1. Treatments included two Putrescine concentrations 10−3 and 10−6 M, and control (C). Error bars represent standard error of the mean (SEM) for three independent replicates.
Fig 9.
Number of carpospores released per day (1-5) in March 2024 after 10 days of acclimatization.
Boxplots represent, from bottom to top, the first quartile, median, third quartile and the maximum values, including outliers.**indicates a statistically significant difference (p < 0.001).