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Fig 1.

Thalamic Segmentation Workflow.

Overview of the principal stages involved in thalamic segmentation using diffusion-weighted imaging (DWI) and T1-weighted (T1w) images, from preprocessing and feature extraction to clustering and label generation.

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Fig 2.

Workflow diagram illustrating clustering.

The thalamus is first divided into small regions using BIRCH clustering for initialization, followed by spectral clustering to produce the final labels.

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Fig 3.

Qualitative Analysis of Thalamic Clustering Algorithms.

Axial (z = 6 mm) and coronal (y = –25 mm) sections on the ICBM 2009b T1-weighted template comparing the Krauth-Morel atlas and thalamic parcellation strategy. For each method, the first column shows the spatial probabilistic label maps, and the second column shows the corresponding maximum-probability label maps.

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Fig 4.

Evaluation of thalamic segmentation accuracy.

(a) Confusion matrices showing Dice scores between the maximum-probability label maps generated by each clustering method and the reference atlases (Krauth–Morel and Allen Brain Human Atlases). (b) Overlay of maximum-probability label maps and atlas labels on an MNI template, illustrating the anatomical correspondence between data-driven clusters and atlas-defined thalamic nuclei.

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Table 1.

Per-nucleus statistical comparison of Dice scores between k-means (nc = 7) and spectral clustering (nc = 9) using Wilcoxon signed ranked test.

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Fig 5.

Qualitative Analysis of Pulvinar Parcellation.

Spatial probabilistic label maps and corresponding maximum-probability label maps projected onto the ICBM 2009b T1-weighted MNI template at the axial slice (z = 6 mm) and coronal slice (y = −29). Results are shown for the Krauth–Morel atlas (left), k-means clustering (center), and spectral clustering (right), illustrating qualitative differences in pulvinar parcellation.

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Fig 6.

Pulvinar Dice Score Analysis Across Reference Atlases.

Dice score confusion matrices between clustering-derived pulvinar subdivisions and the reference atlases, Krauth–Morel (top) and Allen Human Brain Atlas (bottom). Results are shown for k-means (left) and spectral clustering (right). Rows correspond to atlas labels, and columns correspond to cluster labels.

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