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Table 1.

Software requirements and availability.

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Fig 1.

GermVarX workflow.

The GermVarX workflow comprises two primary phases: per-sample processing and cohort-level processing and annotation.

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Fig 2.

GermVarX variant benchmarking.

Benchmarking of GATK-Join, DV-GLN, and Consensus strategies for SNPs and INDELs. DV-GLN and Consensus show highest SNP accuracy, while GATK-Join provides balanced INDEL performance.

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Table 2.

Resource usage across GermVarX pipeline stages. The complete pipeline finished in 77.8 hours of wall-clock time. Reported CPU and wall-clock hours are cumulative task sums across all samples in each stage, not actual elapsed runtime.

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Table 3.

Comparison of GermVarX with existing WES workflows.

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