Fig 1.
Visualization of the main analysis steps: (a) raw microscope image, (b) segmentation, (c) tracking, and (d) linking.
Fig 2.
Evolution of the number of cells in one video over time for control and irradiated conditions.
Exponential growth model fitted to estimate doubling times.
Fig 3.
Lineage trees reconstructed with the CLT algorithm for representative wells under control and irradiated conditions.
(a) Control sample (0 Gy) after 60 h and (b) irradiated sample (5 Gy) after 96 h. Each node represents an individual cell, with node size scaled to its measured surface area. Colors indicate tree size, allowing visualization of differences in clonal expansion between conditions.
Fig 4.
Cell cycle duration for control condition.
Histogram in black and gaussian fit in red (N = 479 cells; Median = 24 h; Std = 9 h). Only cells for which both the mother and daughters were identified were included.
Table 1.
Initial cell numbers and cumulative cell counts across irradiation doses.
Fig 5.
Classification of cells over time into the different categories for control condition 0 Gy and irradiated conditions (1 to 5 Gy).
Proliferative cells in green, transiently arrested trees in orange (ITA cells in dark orange, RPTA in light orange), and LTA cells in red.
Fig 6.
Evolution of the mean individual surface area over time for control condition 0 Gy and irradiated conditions (1 to 5 Gy), for cells from proliferative cell trees.
Errors bars represent this standard deviation of the cell population.
Fig 7.
PTE of surface area of cells from proliferative cell trees for control condition 0 Gy and irradiated conditions (1 to 5 Gy).
Dashed line indicate the median value at the intersection with the curve, while the blue shaded area represents the range of median values across all conditions.
Table 2.
Surface area and diffusion coefficient of proliferating cells.
Fig 8.
PTE of diffusion coefficient of cells from proliferative cell trees for control condition 0Gy and irradiated conditions (1 to 5 Gy).
Dashed line indicate the median value at the intersection with the curve, while the blue shaded area represents the range of median values across all conditions.
Fig 9.
Evolution of the mean individual surface over time for control condition 0Gy and irradiated conditions (1 to 5 Gy); for cells from transliently arrested cell trees.
Errors bars represent this standard deviation of the cell population.
Fig 10.
PTE of surface area of cells from transiently arrested trees, for control condition 0Gy and irradiated conditions (1 to 5 Gy); (a) ITA cell, (b) RPTA cells.
Dashed line indicate the median value at the intersection with the curve, while the blue shaded area represents the range of median values across all conditions.
Table 3.
Surface area and diffusion coefficient of transiently arrested cell trees.
Fig 11.
PTE of diffusion coefficient of cells from transiently arrested cell trees for control condition 0Gy and irradiated conditions (1 to 5 Gy).
(a) ITA cell, (b) RPTA cell. Dashed line indicate the median value at the intersection with the curve, while the blue shaded area represents the range of median values across all conditions.
Fig 12.
Evolution of the mean individual surface area over time for irradiated conditions (1 to 5 Gy) for LTA cells.
Errors bars represent this standard deviation of the cell population.
Fig 13.
PTE of surface area of LTA cells for control condition 0Gy and irradiated conditions (1 to 5 Gy).
Dashed line indicate the median value at the intersection with the curve, while the blue shaded area represents the range of median values across all conditions.
Table 4.
Surface area and diffusion coefficient of LTA cells.
Fig 14.
PTE of diffusion coefficient of LTA cells for control condition 0Gy and irradiated conditions (1 to 5 Gy).
Dashed line indicate the median value at the intersection with the curve, while the blue shaded area represents the range of median values across all conditions.
Fig 15.
Evolution of the mean individual surface area over time of control for proliferative cells (dashed line) and 5 Gy irradiated conditions for all cells categories (solid line): proliferative, transiently arrested (TA) and long-term arrested (LTA).
Errors bars represent this standard deviation of the cell population.
Fig 16.
Median of individual cell surface area for proliferative cells, Initial Transliently Arrested (ITA) cells and Re-Proliferating Transiently Arrested (RPTA) cell, and Long-Term Arrested (LTA) cells from 0 to 5 Gy.
Error bars are estimated using Monte Carlo resampling, see Materials and Methods.
Fig 17.
Median of individual cell diffusion coefficient for proliferative cell trees, Initial Transliently Arrested (ITA) cells and Re-Proliferating Transiently Arrested (RPTA) cell, and Long-Term Arrested (LTA) cells from 0 to 5 Gy.
Error bars are estimated using Monte Carlo resampling, see Materials and Methods.