Fig 1.
Experimental design showing the distribution of groups.
Table 1.
Oligonucleotide sequences of the genes used for RT-qPCR.
Fig 2.
Cellular morphology after 48 hours of cAT-MSCs cultivation.
Fig 3.
Growth curve of mesenchymal stem cells after 144 hours in different cell culture conditions.
Data represent means of cells harvested from 6 animals (p ≤ 0.05).
Fig 4.
Percentage of live cells, in apoptosis or necrosis after the cultivation of mesenchymal stem cells under different experimental conditions (p ≤ 0.05).
Fig 5.
Expression of the pro-apoptotic gene CASP9 in adipose-derived canine mesenchymal stem cells cultured for 48 hours under different conditions.
Bars represent relative mean values normalized to the reference gene GAPDH and standard error of the mean (┬ and ┴). Means denoted by a different letter indicate statistically significant differences among treatments (p < 0.05).
Fig 6.
Expression of the DKC1 gene related to cell survival in adipose-derived canine mesenchymal stem cells cultured for 48 hours under different conditions.
Bars represent relative mean values normalized to the reference gene GAPDH and standard error of the mean (┬ and ┴). Means denoted by a different letter indicate statistically significant differences among treatments (p < 0.05).
Fig 7.
Expression of the FGF2 gene related to proliferation and differentiation in adipose-derived canine mesenchymal stem cells cultured for 48 hours under different conditions.
Bars represent relative mean values normalized to the reference gene GAPDH and standard error of the mean (┬ and ┴). Means denoted by a different letter indicate statistically significant differences among treatments (p < 0.05).
Fig 8.
Expression of the angiogenesis-related gene VEGFA in adipose-derived canine mesenchymal stem cells cultured for 48 hours under different conditions.
Bars represent relative mean values normalized to the reference gene GAPDH and standard error of the mean (┬ and ┴). Means denoted by a different letter indicate statistically significant differences among treatments (p < 0.05).
Fig 9.
Expression of the cell cycle regulator gene TP53 in adipose-derived canine mesenchymal stem cells cultured for 48 hours under different conditions.
Bars represent relative mean values normalized to the reference gene GAPDH and standard error of the mean (┬ and ┴). Means denoted by a different letter indicate statistically significant differences among treatments (p < 0.05).
Fig 10.
Expression of the HIF1 gene, a cellular regulator of the adaptive response to hypoxia, in adipose-derived canine mesenchymal stem cells cultured for 48 hours under different conditions.
Bars represent relative mean values normalized to the reference gene GAPDH and standard error of the mean (┬ and ┴). Means denoted by a different letter indicate statistically significant differences among treatments (p < 0.05).
Fig 11.
Amount of proteins identified in Canis lupus familiaris MSCs (http://bioinformatics.psb.ugent.be/webtools/Venn/).
Fig 12.
Dendrogram and principal components analysis.
(A, C and E) Dendrogram of emPAIs of proteins of the control group, versus DFO, IFN-γ + DFO e INF-γ. (B, D and F) Analysis of the components of the control group, versus DFO, IFN-γ + DFO e INF-γ.
Fig 13.
Differential protein abundance of MSCs from the control group versus DFO (t-test, P < 0.01*, Fisher’s LSD test, FDR < 0.05).
Fig 14.
Differential protein abundance of MSCs from the control group versus IFN-γ + DFO (t-test, P < 0.01*, Fisher’s LSD test, FDR < 0.05).
Fig 15.
Differential protein abundance of MSCs from the control group versus IFN-γ (t-test, P < 0.01*, Fisher’s LSD test, FDR < 0.05).