Table 1.
Primers used in this study.
Fig 1.
Sequence alignment of Sfp-type PPTases.
The protein sequences used for alignment are as follows: Pseudomonas plecoglossicida NB2011 PPTase, Pseudomonas aeruginosa Pcps (WP_003112468.1), Salmonella enterica typhimurium EntD (AAA91937.1), Bacillus substilis Sfp (ACG68433), and Bacillus cereus Sfp (WPQ43272.1). Conserved amino acids are shaded in black; gray shading indicates that 100% of the residues are similar at that position. The symbol “*” indicates a number 10 less than the following number.
Fig 2.
Growth curves of mutant and wild type strain.
Measure the strain concentration within 14h under the condition of OD600 and draw the growth curve diagram.
Fig 3.
Swarming and swimming ability of the mutant and wild type strain.
A, Swarming motility of the mutant and WT; B, Swimming ability of the mutant and WT. Data was mean±SD of three independent experiments.
Fig 4.
Lysine autotrophy observation, sensitivity to H2O2 and siderophore activity of the mutant and WT strain.
A, lysine autotrophy observation, after 12 hours, the OD600 of the bacterial culture was measured; B, sensitivity to H2O2 treatment; C, siderophore activity of the mutant and WT strain, after 24 hours cultivation, the culture supernatants were collected and siderophore activity measured; D, Csaky test for hydroxamte siderophore of the mutant and WT strain, the 24h hours culture supernatant was detected by Csaky test for hydroxamate type of siderophores. A, B, C, Data was mean±SD of three independent experiments. Asterisk “*” and “**” denotes a significant difference at the level of P < 0.05 and P < 0.01, respectively. D, Representative data was shown from three independent replicate tests.
Table 2.
Fatty acid profiles of P. plecoglssicida NB2011 and the mutant ΔPPT::EntD.
Fig 5.
Adhesion, internalization and replication of the mutant and WT strain in the macrophage J774A.1.
CFU level of the strains at −3 h (adhesion), 0 h (internalization) and 0-24 h (replication), respectively. The data is presented as mean ± standard deviation (SD) from 3 replicates for each sample.
Table 3.
Virulence test of the mutant and wild-type strain against the large yellow croaker.
Table 4.
Differently expressed genes (DEGs) in the mutant ΔPPT::EntD P. plecoglossicida.