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Table 1.

Microscopic studies of Oidiodendron maius from different sources.

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Table 1 Expand

Table 2.

Biochemical tests for different strains of Oidiodendron maius.

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Table 2 Expand

Fig 1.

Oidiodendron maius colony on SDA media.

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Fig 1 Expand

Table 3.

Selected mutants of Oidiodendron maius after screening and selection.

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Table 3 Expand

Table 4.

Analysis of variance table for optimization of pH for fibrinolytic enzyme production from wild and mutated strains of Oidiodendron maius.

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Table 4 Expand

Fig 2.

Comparative enzyme production from wild-type and mutated Oidiodendron maius strains across A) varying pH values B) varying temperatures C) different substrate concentrations D) different inoculum sizes E) different fermentation periods F) Bradford standard curve for Oidiodendron maius.

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Fig 2 Expand

Table 5.

Analysis of variance table for optimization of temperature for fibrinolytic enzyme production from wild and mutated strains of Oidiodendron maius.

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Table 5 Expand

Table 6.

Analysis of variance for optimization of substrate concentration (%) for fibrinolytic enzyme production by wild-type and mutated Oidiodendron maius strains.

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Table 7.

Analysis of variance for optimization of inoculum size for fibrinolytic enzyme production by wild-type and mutated Oidiodendron maius strains.

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Table 7 Expand

Table 8.

Analysis of variance for optimization of fermentation period for fibrinolytic enzyme production by wild-type and mutated Oidiodendron maius strains.

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Table 9.

Purification summary of fibrinolytic enzyme produced by wild-type and UV-mutated Oidiodendron maius Strains, Gamma mutated Oidiodendron maius, Ethidium bromide mutated Oidiodendron maius and nitrous acid mutated Oidiodendron maius.

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Fig 3.

Ammonium sulphate precipitation of fibrinolytic enzyme produced by A) wild-type Oidiodendron maius strain B) UV mutated Oidiodendron maius strain C) gamma mutated Oidiodendron maius strain D) Ethidium Bromide mutated Oidiodendron maius strain E) nitrous acid mutated Oidiodendron maius strain.

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Fig 3 Expand

Fig 4.

SDS-PAGE evaluation of fibrinolytic enzymes produced by BBTI-EB-150 mutated Oidiodendron maius.

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Fig 4 Expand

Fig 5.

Fibrinolytic activity of enzyme produced by BBTI-EB-150 mutant strain.

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Fig 5 Expand

Table 10.

CaCl2 Induced clotting time assay for fibrinolytic enzymes from Oidiodendron maius.

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Table 10 Expand

Fig 6.

CaCl2-induced clotting time assay for ethidium bromide mutant strain (BBTI-EB-150) of Oidiodendron maius.

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Fig 6 Expand

Fig 7.

A) Effect of pH on enzyme activity from wild-type strain of Oidiodendron maius B) Effect of pH on enzyme activity from Ethidium bromide strain of Oidiodendron maius C) Effect of temperature on enzyme activity from wild strain of Oidiodendron maius D) Effect of temperature on enzyme activity from Ethidium bromide mutated strain of Oidiodendron maius.

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Fig 7 Expand

Table 11.

Kinetic and thermodynamic parameters for irreversible thermal inactivation of fibrinolytic enzyme derived from BBTI-EB of Oidiodendron maius.

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Table 11 Expand