Fig 1.
Violin plots representation of distribution between parameters for all the identified O-glycopeptide sequences.
A. Monoisotopic molecular weight (Ma Cal). B. Ion score. C. Peptide length. Each plot displays the distribution density and individual peptide values.
Fig 2.
Functional enrichment of identified O-GlcNAcylated proteins identified in a C. elegans model of ageing and AD.
A) STRING-based PPI analysis of O-GlcNAcylated proteins identified in L1 stage and adult worms of the WT N2 strain (based on 21 first-shell interactions). B) STRING-based PPI analysis of O-GlcNAcylated proteins identified in L1 stage, and adult worms of the aex-3p::Tau(V337M) strain (based on one first-shell interaction). C) STRING-based PPI analysis of O-GlcNAcylated proteins identified in L1 stage, and adult worms of the aex-3p::Tau(V337M) strain (based on a second-shell expansion for a total of 21 1st- and 2nd-shell interactions). Proteins identified with a blue halo represent those with high and very high confidence (ion score and pExpect: < 0.05).
Fig 3.
Bubble charts of enrichment terms and hypothetical schematic of non-canonical protein O-GlcNAcylation of an ER-resident protein.
A Biological process. B) Molecular function. C) Cellular component. D) Reactome Pathways. E) Protein Domains and features enrichment in the WT N2 strain. F) KEGG Pathways enrichment in the aex-3 transgenic strain). G) Non-canonical O-GlcNAcylation: Relocated PDI-2 (UniProt: PDI2_CAEEL) modified in cytosol).