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Table 1.

Location, number of samples, surface material, and porosity classification of sampled surfaces in porcine reproductive and respiratory syndrome positive growing pig farms selected for the study.

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Table 1 Expand

Table 2.

Proportion of positive surface samples by standard-RT-qPCR and viability-RT-qPCR, oral fluids by RT-PCR per farm, state location, biosecurity feature, and porcine reproductive and respiratory syndrome virus classification.

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Table 2 Expand

Table 3.

Number of standard-RT-qPCR and viability-RT-qPCR porcine reproductive and respiratory syndrome virus positive surface samples by material and porosity classification.

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Table 3 Expand

Table 4.

Number of porcine reproductive and respiratory syndrome virus positive standard-RT-qPCR and viability-RT-qPCR surface samples and median cycle threshold values by location.

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Table 4 Expand

Fig 1.

Surface standard-RT-qPCR and viability-RT-qPCR results by location and farm.

Surface was either negative to both S-RT-qPCR and V-RT-qPCR (green), positive for S-RT-qPCR (red), or positive for both S-RT-qPCR and V-RT-qPCR (orange).

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Fig 1 Expand

Fig 2.

Distribution of cycle threshold value results by assay and location.

Assay type was either S-RT-qPCR (orange) or V-RT-qPCR (purple).

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Fig 2 Expand

Fig 3.

Distribution of cycle threshold value results by assay, surface material, and porosity classification.

Assay type was either S-RT-qPCR (orange) or V-RT-qPCR (purple).

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Fig 3 Expand

Table 5.

Cohen’s kappa and global agreement of detection of porcine reproductive and respiratory syndrome between two assays.

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Table 5 Expand