Table 1.
Oligonucleotides for specific primer.
Fig 1.
Comparison of the P. argenteus Ars and Ers protein sequences with other animals.
(A) Phylogenetic relationship of androgen receptor (Arα/Arβ) proteins from P. argenteus with other animals. (B) Phylogenetic relationship of estrogen receptor (Esr1/Esr2a/Esr2β) proteins from P. argenteus with other animals.
Fig 2.
The tissue distribution expression of arα and arβ in female and male fish.
(A and B) The tissue distribution expression of arα in female and male fish, respectively (n = 3). (C and D) The tissue distribution expression of arβ in female and male fish, respectively (n = 3). Superscript letters indicate one-way ANOVA, followed by Duncan’s test (P < 0.05). “N.D.” indicate not detect by qPCR.
Fig 3.
The tissue distribution expression of esr1, esr2a and esr2β in female and male fish.
(A and B) The tissue distribution expression of esr1 in female and male fish, respectively (n = 3). (C and D) The tissue distribution expression of esr2a in female and male fish, respectively (n = 3). (E and F) The tissue distribution expression of esr2β in female and male fish, respectively (n = 3). Superscript letters indicate one-way ANOVA, followed by Duncan’s test (P < 0.05).
Fig 4.
Profiles of gonadal development in P. argenteus.
The ovarian development in different stages, including stage I (A), stage II (B), stage III (C), stage IV (D), stage V (E), and stage VI (F). The testicular development in different stages, including stage I (G), stage II (H), stage III (I), stage IV (J), stage V (K), and stage VI (L). OG, oogonia; PO, primary oocyte; VO, vitellogenic oocyte; Fc, follicle cells; RO, regressed oocyte. SGA, spermatogonia type A; SGB, spermatogonia type B; PSC, primary spermatocyte; SSC, secondary spermatocyte; SP, sperm.
Fig 5.
The expression of arα and arβ in gonad during gonadal development.
(A) The expression of arα during ovarian developmental stages (n = 3). (B) The expression of arα during testicular developmental stages (n = 3). (C) The expression of arβ during ovarian developmental stages (n = 3). (D) The expression of arβ during testicular developmental stages (n = 3). Superscript letters indicate one-way ANOVA, followed by Duncan’s test (P < 0.05).
Fig 6.
The expression of esr1, esr2a and esr2β in gonad during gonadal development.
(A) The expression of esr1 during ovarian developmental stages (n = 3). (B) The expression of esr1 during testicular developmental stages (n = 3). (C) The expression of esr2a during ovarian developmental stages (n = 3). (D) The expression of esr2a during testicular developmental stages (n = 3). (E) The expression of esr2β during ovarian developmental stages (n = 3). (F) The expression of esr2β during testicular developmental stages (n = 3). Superscript letters indicate one-way ANOVA, followed by Duncan’s test (P < 0.05).
Fig 7.
The expression of arα in pituitary during gonadal development.
(A) The expression of arα in pituitary during ovarian development (n = 3). (B) The expression of arβ in pituitary during testicular development (n = 3). Superscript letters indicate one-way ANOVA, followed by Duncan’s test (P < 0.05).
Fig 8.
The expression of esr1, esr2a and esr2β in pituitary during gonadal development.
(A) The expression of esr1 in pituitary during ovarian development (n = 3). (B) The expression of esr1 in pituitary during testicular development (n = 3). (C) The expression of esr2a in pituitary during ovarian development (n = 3). (D) The expression of esr2a in pituitary during testicular development (n = 3). (E) The expression of esr2β in pituitary during ovarian development (n = 3). (F) The expression of esr2β in pituitary during testicular development (n = 3). Superscript letters indicate one-way ANOVA, followed by Duncan’s test (P < 0.05).
Fig 9.
Effects of different treatments on ars expression in gonadal tissue.
The expression of arα was analyzed by qPCR (n = 6 for each value) after different hormones treatment in ovarin tissue (A) and testicular tissue (B). The expression of arβ was analyzed by qPCR (n = 6 for each value) after different hormones treatment in ovarin tissue (C) and testicular tissue (D). Superscript letters indicate one-way ANOVA, followed by Duncan’s test (P < 0.05).
Fig 10.
Effects of different treatments on ers expression in gonadal tissue.
The expression of esr1 was analyzed by qPCR (n = 6 for each value) after different hormones treatment in ovarin tissue (A) and testicular tissue (B). The expression of esr2a was analyzed by qPCR (n = 6 for each value) after different hormones treatment in ovarin tissue (C) and testicular tissue (D). The expression of esr2a was analyzed by qPCR (n = 6 for each value) after different hormones treatment in ovarin tissue (E) and testicular tissue (F). Superscript letters indicate one-way ANOVA, followed by Duncan’s test (P < 0.05).