Table 1.
Plackett- Burman experimental matrix for screening key variables affecting collagenase synthesis.
Table 3.
CCD-RSM design matrix for significant parameters influencing collagenase synthesis.
Fig 1.
Phylogenetic tree utilizing the neighbour joining technique based on the 16S rRNA gene sequences of isolated bacterial strain Z1.
Fig 2.
Collagenase production kinetics and effect of physio-chemical factors on collagenase production (A) production kinetics, (B) inoculum size, (C) effect of metal ions, (D) effect of carbon sources, (E) effect of nitrogen sources, (F) effect of pH and (G) effect of temperature.
The data values are the average of three replicates, while the error bars indicate standard deviation.
Table 2.
Analysis of variance for PBD for collagenase production.
Table 4.
Analysis of variance for quadratic model of CCD-RSM for collagenase production.
Fig 3.
Response surface plot of collagenase production.
Results show significant interactions among identified parameters at p < 0.05.
Fig 4.
UV -Visible spectroscopic characterization of nanoparticles (A) silver nanoparticles (AgNP) at 5mM, 10 mM and 50 mM and (B) zinc oxide nanoparticles (ZnONP) at 10mM and 50mM.
Fig 5.
Morphological and compositional analysis of Collagen hydrolysate capped nanoparticles(A) FTIR analysis of AgNP, (B) FTIR analysis of ZnONP, (C) SEM-EDS analysis of AgNP, (D) SEM-EDS analysis of ZnONP, (E) XRD analysis of AgNP, (F) XRD analysis of ZnONP, (G) TGA analysis of AgNP, (H) TGA analysis of ZnONP, (I) AFM analysis of AgNP and (J) AFM analysis of ZnONP.
Table 5.
Antimicrobial activity assessment of collagenase enzyme and its Nanoparticles.
Fig 6.
Antioxidant activity of collagen hydrolysate and nanoparticles using(A) ABTS assay and (B) DPPH radical scavenging activity.
Fig 7.
Assessment of anticancer potential of nanoparticles and collagen hydrolysates against breast cancer cells.