Fig 1.
Overall Experimental Flowchart.
Fig 2.
Reverse liquid chromatography analysis.
The chromatogram on the left shows the original Primovist solution, containing two adjacent absorption peaks corresponding to the Gd-A and Gd-B isomers. The middle and right chromatograms display single, pure peaks for the isolated Gd-A and Gd-B isomers, respectively, confirming successful separation.
Fig 3.
Solid powder of pure Gd-A and Gd-B.
The concentrated pure Gd-A and Gd-B were converted into solid powder using a freeze-drying machine and sealed in glass vials for storage, with each vial containing approximately 104.25 mg.
Fig 4.
Dynamic MRI Imaging of Gd-A and Gd-B in the Rabbit Gallbladder.
a. Following intravenous injection of Gd-A, the liver signal intensity significantly increased, with a small amount of contrast agent appearing in the gallbladder at 5 minutes (indicated by the arrow). b. Following intravenous injection of Gd-B, the liver signal intensity was relatively weaker, with a small amount of contrast agent appearing in the gallbladder at 15 minutes (indicated by the arrow).
Fig 5.
MRI Contrast-Enhanced Signal Time Curve.
The liver MRI time-signal curves for Groups A and B were obtained by performing intravenous bolus injection at 2 minutes after the start of scanning, followed by continuous T1-weighted dynamic contrast-enhanced imaging without intervals.
Fig 6.
Exponential Decay Fitting of Blood Drug Concentration and Calculation of Area Under the Curve.
Table 1.
Changes in Purity of Gd-A and Gd-B at Different Temperatures.
Table 2.
MR Imaging and Pharmacokinetic Results.
Fig 7.
Comparison of the Distribution of Hepatic Pharmacokinetic Parameters.
The figures include (in order) box plots of peak time in liver parenchyma, box plots of peak signal intensity in liver parenchyma, box plots of plasma clearance rate, and box plots of half-life.
Fig 8.
Sturctures of gadoxetate geometrical isomers.
Sturctures of gadoxetate geometrical isomers in 4S,6R/right-handed, 4S,6R/left-handed, 4S,6S/left-handed, 4S,6S/right-handed forms.