Fig 1.
A brief flow diagram of this study.
Table 1.
Index of selected CD8+ epitopes of aminopeptidase, heat shock protein, P23 protein, serine protease and sporozoites glycoprotein with their antigenic, non-allergenic and non-toxic properties.
Table 2.
Index of the best CD4+ epitopes from aminopeptidase, heat shock protein, P23 protein, serine protease and sporozoites glycoprotein with their antigenic, allergenic and toxic properties.
Table 3.
Index of the best continuous B-cell epitopes with their toxic, allergenic and antigenic properties.
Fig 2.
The multiepitope mRNA vaccine construct against C. hominis and C. hominis with adjuvant (green), epitopes (CD8 + /MHC-1-paste, CD4 + /MHC/2-blue, continuous/linear B-cell-golden), and linkers (EAAAK-yellow, AYY-sky blue, AK-violet, KFER-red).
Table 4.
Biophysical characteristics of the vaccine.
Fig 3.
The PSIPRED generated two-dimensional structure of vaccine (A) where the initial bar (Conf) shows the confidence level in the forecast; different sizes of the bar indicate different degrees of confidence.
Yellow represents the beta-sheet, pink the helix, and grey the vaccine’s coil structure in the second bar (Cart). The three separate amino acid sequences and structural aspects are represented by the third (Pred) and fourth (AA) bars, respectively. Additionally, the amount of the helix, sheet, coil and turn by GOR4 (B) and SOPMA (C) servers.
Fig 4.
Prediction and validation of the predicted three-dimensional model of the vaccine with (A) non-refined structure (B) refined structure, (C). Z-score, (D) Ramachandran plot scores and (E) ERRAT score.
Fig 5.
Three-dimensional illustration of discontinuous B-cell epitopes of the vaccine (A–K).
Yellow-color showcases the discontinuous B cell epitopes and the grey sticks represents the vaccine.
Table 5.
The energy values and bonding features between the ligand-receptor.
Fig 6.
The interaction between the vaccine construct and TLR-2 (A) and TLR-4 (B).
Fig 7.
The analysis of the dynamic simulation of molecules for both vaccine-TLR-2 and vaccine-TLR-4 complexes.
A representation of (A) covariance graph (correlated, uncorrelated, and anti-correlated motions are represented by red, white, and blue regions, respectively, (C) network of elasticity, (E) B factor, (G) deformability, (I) eigenvalue, and (K) the variance of vaccine-TLR-2 complex. There exists another representation of (B) covariance graph (correlated, uncorrelated, and anti-correlated motions are represented by red, white, and blue regions, respectively, (D) network of elasticity, (F) B factor, (H) deformability, (J) eigenvalue, (L) variance of vaccine-TLR-4 complex.
Fig 8.
Cloning of the vaccine construct.
The depiction was about the (A) vector, (B) DNA of sequence of the vaccine and (C) cloned construct of the vaccine.
Fig 9.
The immune simulation of the vaccine, where the illustration is depicted as (A) The lifespan of B-cell, (B) TH-cell, (C) TR-cell, (D) NK cell, (E) DC cell and (F) MA cell.
Fig 10.
The predicted mRNA structures of the vaccine by the RNAfold web server.
The centroid structures of the vaccine with the base pair probabilities (A and B) and the positional entropy (C and D).