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Table 1.

E. Intestinalis extract GCMS Peak Report TIC.

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Fig 1.

GCMS profile of Enteromorpha intestinalis extract.

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Fig 2.

UV- spectral presentation of E. intestinalis extract (0.2%) in coconut and olive oil.

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Fig 3.

Pseudoternary phase diagram of olive oil with Smix.

(Tween 80: PEG) and water at ratio of 1:1, 2:1, and 3:1.

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Table 2.

Composition and characterization of E. intestinalis extract loaded-nanoemulsions computed by simplex lattice design.

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Fig 4.

The visual appearance of nanoemulsion formulations (F1-F7).

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Table 3.

Summary of the model statistics.

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Fig 5.

3D surface plots demonstrating the effect of the independent variables; oil (A), Smix (B), and water (C) and dependent variables; globule size (nm), PDI, and Viscosity.

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Table 4.

Stability studies of nanoemulsion formulation (F6) at different storage temperature.

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Table 5.

Droplet size and PDI of F6 nanoemulsion formulation at various time intervals.

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Fig 6.

Measurement of the Droplet size of E. intestinalis based-NE at various time intervals.

(Left panel) DLS results of NE illustrating the experimental conditions, i.e., the mean autocorrelation function, monodispersity and radius, respectively. (Right panel) Comparative corresponding droplet size of NF at various time intervals (from top to bottom panel, respectively). All experiments were performed with an auto-piloted run of 50 measurements at every 20 s, with a wait time between of 1 s (at 25 °C).

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Fig 7.

continued.

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Table 6.

Antioxidant activity of E. intestinalis extract and F6 nanoemulsion.

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Table 7.

Anti-inflammatory effect of F6 nanoemulsion on Paw edema.

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Fig 8.

Anti-inflammatory effect of nanoemulsion formulation F6 on Paw edema (C; Control, DS; Diclofenac sodium, EI-NE; E. intestinalis nanoemulsion).

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Fig 9.

Acute dermal toxicity assessment on rats’ skin.

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