Fig 1.
Characterization of OPCs by immunocytochemistry.
(A) PDGRFα expression in C-OPCs, (B) Olig2 expression in C-OPCs, (C) PDGRFα expression in L-OPCs, (D) Olig2 expression in L-OPCs (scale bar = 20 µm). (E) Chart of quantitative analysis of PDGFRα and Olig2 markers in isolated OPCs from the CPZ and LPS model (n = 3), (F) Chart of quantitative analysis of SOX10 expression in the normal OPCs and isolated OPCs from the CPZ and LPS model (n = 3). Means ± SD are used to define all data. (ns: non-significant); *P < 0.05, **P < 0.01, and ***P < 0.001.
Fig 2.
DiI-labeled OPCs in the corpus callosum and visceral organs (lung, liver, and spleen) 24 h after transplantation.
Results of the CC showed C-OPC homing was higher than L-OPCs: (A) CC of the CPZ + C-OPCs group, (B) CC of the CPZ + L-OPCs group (scale bar = 50 µm). Also, results of the liver and spleen showed trapped C-OPCs were less than L-OPCs: (C) liver of the CPZ + C-OPCs group, (D) liver of the CPZ + L-OPCs group, (E) spleen of the CPZ + C-OPCs group, and (F) spleen of the CPZ + L-OPCs group (scale bar = 50 µm). No OPCs showed in the lung: (G) lung of the CPZ + C-OPCs group, (H) lung of the CPZ + L-OPCs group (scale bar = 50 µm). (I) Chart of quantitative analysis of homing OPCs in the CC (n = 4). (J) Chart of quantitative analysis of trapped OPCs in the lung, liver, and spleen (n = 3). Means ± SD are used to define all data. *P < 0.05, **P < 0.01, and ***P < 0.001.
Fig 3.
Evaluation of myelinating area in the corpus callosum by LFB staining.
Results showed complete myelin content (blue color) in healthy and decreased myelin content in the chronic CPZ group (white color); after OPC transplantation, the myelin content increased in CPZ + C-OPCs & CPZ + L-OPCs; also, the increase was higher in CPZ + C-OPCs; (A) Healthy CC, (B) CC of Chronic CPZ group, (C) CC of CPZ + C-OPCs group, (D) CC of CPZ + L-OPC group (scale bar = 100 µm), (E) Mouse coronal brain atlas of Paxino. (F) Chart of quantitative analysis of the remyelination area in the corpus callosum for all groups (n = 3). Means ± SD are used to define all data. *P < 0.05, **P < 0.01, and ***P < 0.001.
Fig 4.
Evaluation of CSPG4 level in the corpus callosum by IF.
Results showed an increase in the CPZ group; also, after transplantation, CSPG4 decreased in both the CPZ + C-OPCs group and the CPZ + L-OPCs group but significantly more in the CPZ + C-OPCs group compared with others: (A) chronic CPZ group, (B) CPZ + C-OPCs group, (C) CPZ + L-OPCs group (scale bar = 50 µm). (D) Chart of quantitative analysis of CSPG level in the corpus callosum for all groups (n = 3). Means ± SD are used to define all data. *P < 0.05, **P < 0.01, and ***P < 0.001.
Fig 5.
Evaluation of (A) MBP, (B) MOG, and (C) TGFβ mRNA expression by RT-qPCR and quantitative analysis.
Results showed significantly higher levels in the CPZ + C-OPCs group compared with the CPZ & CPZ + L-OPCs groups (n = 3). Means ± SD are used to define all data. (ns: non-significant); *P < 0.05, **P < 0.01, and ***P < 0.001.