Table 1.
Summary Statistics Using Cq Values for Each Primer Pair.
Fig 1.
Melt curve temperature plots for all in group samples.
Single source specimens for plants (A), fungi (B), bacteria (C), and arthropods (D) underwent melt curve analysis for specificity testing of each primer pair. Each trace is a different sample. The y-axis is the derivative reporter fluorescence (-Rn’) and the x-axis is temperature (°C). The peak of the melt curve is the temperature at which 50% of DNA is double stranded and 50% is single stranded.
Table 2.
Reference Intervals for Each Primer Pair Based on Melt Curve Temperatures (°C).
Table 3.
Regression Analysis of gBlock™ Standard Curves.
Table 4.
Sensitivity Assessment Utilizing the Lowest Standard of Each Primer Pair.
Table 5.
Summary of in silico analysis using ecoPCR.