Fig 1.
Flowchart of the study protocol.
Table 1.
Comparison of demographic characteristics among groups.
Fig 2.
Phylum-level composition and differential analysis.
(a) relative abundance of top 20 phyla in four groups. (b) Abundance variation of Bacillota. (c-f) Pairwise comparisons of differentially abundant phyla between groups: (c) N vs H, (d) H vs T, (e) T vs CIN, and (f) N vs CIN.
Fig 3.
Genus-level composition and differential analysis.
(a) relative abundance of top 10 genera in four groups. (b) Abundance variation of Lactobacillus. (c-f) Pairwise comparisons of differentially abundant genera between groups: (c) N vs H, (d) H vs T, (e) T vs CIN, and (f) N vs CIN.
Fig 4.
The microbial diversity analysis in different groups.
(a) Ace index box chart for each group. (b) Sob index box chart for each group. (c) Chao1 index box chart for each group.(*P < 0.05,**P < 0.01). (d) The microbial Beta diversity analysis in different groups.
Fig 5.
A heatmap of Spearman correlation coefficients between the top 10 genera and clinical factors.
(* 0.01 < P ≤ 0.05,**0.001 < P ≤ 0.01,***P ≤ 0.001).
Fig 6.
Random Forest and ROC Analysis.
(a) Random-forest graph of top 50 different bacterial communities between each group. (b) Model evaluation with ten-fold cross-validation using varying numbers of important features. (c) ROC curve of Group N. (d) ROC curve of Group H. (e) ROC curve of Group T. (f) ROC curve of Group CIN.