Fig 1.
Project workflow.
Table 1.
Upper, middle, and lower site information at Harris Spring and Deer Creek Spring.
Table 2.
Comparison of water chemistry parameters between HS and DCS. Means ± SD were calculated across all sites and sampling dates within each spring. F-values (numerator df = 1, denominator df ≈ 16) and p-values were obtained from mixed-effects ANOVA models with Spring as a fixed effect and Site nested within Spring as a random effect. Significant differences (p < 0.05) are shown in bold.
Fig 2.
Rarefaction curves for eDNA acquired at all three locations (lower, middle, and upper) at HS and DCS.
Does not include controls (filters pumped with air).
Fig 3.
Box and whisker plots showing inverse Simpson index for all locations at HS and DCS.
Fig 4.
Non-metric multidimensional scaling plot with 95% confidence ellipses separated by spring (DCS as red; HS as blue) and location (lower with a circle, middle with an x, and upper with a triangle).
Fig 5.
Non-metric multidimensional scaling plots with 95% confidence ellipses separated by location (lower, middle, and upper) at HS and DCS.
Fig 6.
Absolute abundance of bacteria and archaea by phylum found at each location at each spring (HS refers to Harris Spring and DC refers to Deer Creek in the sample names).