Table 1.
Overview of molecular and phenotypic results in enterococci.
Fig 1.
Induction of resistance in van gene containing enterococci.
Fig 2.
Relative vanB RNA expression per isolate per condition.
Shown are the relative expression levels of vanB RNA relative to recG RNA for three Enterococcus faecium isolates, each in its vancomycin susceptible phenotype (red colors) and its vancomycin resistant phenotype (blue colors), when these isolates were cultured near (circles) or far away (squares) from a vancomycin disk (5 μg). The y-axis shows the difference in Ct values between the vanB PCR and the recG PCR. ΔCt values <0 indicate higher responsiveness of vanB expression while ΔCt values >0 indicate stronger response of recG than vanB. Results are plotted as mean ± 95% confidence interval.
Fig 3.
Vancomycin binding in phenotypically resistant (blue) and susceptible (red) E. faecium isolates ((N = 2).
Different levels of vancomycin-associated fluorescence is seen between the isolates and phenotypes. Resistant isolates bound half the number of BODIPY™-vancomycin molecules relative to their vancomycin susceptible counterpart (52.8% ± 2.1%).