Fig 1.
Map showing collection sites of ticks in different areas of the selected districts.
Table 1.
List of primers used for the amplification of various genes fragments in the present study.
Table 2.
Spatial and host distribution of ticks infesting dogs and molecular detection of Ehrlichia spp. in Khyber Pakhtunkhwa.
Fig 2.
Collection of Rhipicephalus tick species from stray dogs in this study from district Mardan and Dir Lower.
Fig 3.
A Bayesian Inference phylogenetic tree was constructed on the basis of 16S rDNA sequences of Rhipicephalus spp.
The Ixodes ricinus 16S rDNA sequence was used as an outgroup. The bootstrap supporting values (60-100%) are indicated at each node. The obtained sequences are represented in blue font and accession number PX280287 (Rhipicephalus sp.) and PX258040 (Rhipicephalus haemaphysaloides).
Fig 4.
A Bayesian Inference phylogenetic tree was constructed on the basis of cox1 sequences of Rhipicephalus spp.
The Ixodes ricinus cox1 sequence was used as an outgroup. The bootstrap supporting values (60-100%) are indicated at each node. The obtained sequences are represented in blue font and accession number PX259820 (Rhipicephalus sp.) and PX312474 (Rhipicephalus haemaphysaloides).
Fig 5.
A Bayesian Inference phylogenetic tree was constructed on the basis of 16S rDNA sequences of bacterial species.
The Wolbachia pipientis 16S rDNA sequence was used as an outgroup. The bootstrap supporting values (60-100%) are indicated at each node. The obtained sequences are represented in blue font and accession numbers: PX258218 (Ehrlichia sp.) and PX395829 (Ehrlichia minasensis).
Fig 6.
A Bayesian Inference phylogenetic tree was constructed on the basis of dsb sequences of Ehrlichia spp.
The Ehrlichia sp. dsb sequence was used as an outgroup. The bootstrap supporting values (72-100%) are indicated at each node. The obtained sequences are represented in blue font and accession numbers: PX418075 (Ehrlichia sp.), PX418076 (Ehrlichia sp.) and PX418077 (Ehrlichia minasensis).