Fig 1.
Cell Type Annotation and Gene Expression Analysis of Single-Cell RNA Sequencing Data.
A. UMAP visualization of single-cell data. B. Projected Expression of Immune Cell Subtypes on UMAP. C. Bubble chart of gene expression across 11 cell subtypes. D. The proportion of cell subpopulations in different datasets.
Fig 2.
Intercellular Crosstalk and Gene Expression Pattern Analysis.
A. Intercellular communication between different cell types. B. Connectivity of Different Cell Types in Networks. C. Bubble chart illustrating the significance of communication between different cell types. D. Enrichment Heatmaps and Annotations for Average Expression Across Cell Subpopulations.
Fig 3.
Comparative Analysis of Single-Cell RNA Sequencing Data from Disease and Control Groups.
A. Key genes for burn injury identified by random forest screening. B-C. LASSO Regression Analysis. D-E. Immune Cell Proportion and Correlation Heatmaps Among Different Patients. F. Boxplots of Differences in Immune Cell Subpopulations Between Disease and Healthy Control Groups. G. Correlation of TF, CCL7, and AP2A2 Expression in Immune Cells.
Fig 4.
Correlation Between Key Genes and Different Cytokines.
A. Correlation of TF, CCL7, and AP2A2 with Chemokines. B. The correlation between TF, CCL7, and AP2A2 with receptors. C. The relationship between TF, CCL7, and AP2A2 with the Major Histocompatibility Complex. D. Correlation between TF, CCL7, and AP2A2 with Immunosuppressive Factors. E. Correlation between TF, CCL7, and AP2A2 with Immunostimulatory Factors.
Fig 5.
Enrichment Analysis Results of Key Genes.
A-C. GSEA and GSVA enrichment analysis results for AP2A2, CCL7, and TF.
Fig 6.
Association Between Gene Expression Analysis and Cell Type Function.
A. Gene Expression and Correlation Analysis. B. Network diagram of miRNA interactions with AP2A2, CCL7, and TF. C, D. Visualization of Key Genes and Transcription Factors. E, F. Single-cell analysis of key genes reveals significant expression of key genes in macrophages.
Fig 7.
Co-expression Network of Key Genes.
A. Co-expression Network of TLR4 and AP2A2. B. Co-expression Network of IL10 and AP2A2. C. Co-expression Network of TGFB1 and CCL7. D. Co-expression Network of NFKB1 and CCL7.
Fig 8.
Association Analysis Between Gene Expression and Drugs.
A. Enrichment analysis results of key genes (AP2A2, CCL7, TF) across different biological pathways. B. Sanky Diagrams of AP2A2, CCL7, TF with Various Compounds.
Fig 9.
Flowchart.