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Table 1.

Specimen list and information of DNA extraction.

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Fig 1.

DNA extraction result.

A. Extracted DNA concentration of two lysis settings from grasshopper specimens. P and E represents Pretreatment and non-pretreatment employment. Gray lines connect extractions from the two legs of the same specimen; B. The relationship between specimen age and the peak length of DNA fragments extracted. The blue line is the fitted linear regression, and the shaded area indicates the 95% confidence interval. C–D. Specimen 17STF before (C) and after extraction (D).

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Fig 2.

Contamination of DNA.

A. Percentage of exogenous reads across specimens. Different colors represent different phylum. B. Relationship between specimen age and the percentage of exogenous DNA. The blue line is the fitted linear regression, and the shaded area indicates the 95% confidence interval.

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Fig 3.

DNA filtering result.

Percentage of reads (A) and bases (B) retained after filtering with FASTP. Red (cyan) line represents default (custom) setting. Percentage of reads contains polyG (C).

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Fig 3 Expand