Table 1.
Morphological, physiological and biochemical characters of Streptomyces coelicolor.
Fig 1.
Phylogenetic tree depicting the evolutionary relationships of S. coelicolor based on 16S rRNA partial gene sequences comparison following Neighbor-Joining method.
Table 2.
Determination of the ligand denticity of hydroxamate siderophore produced by S. coleicolor.
Fig 2.
FT-IR spectrum illustrating the functional groups present in the siderophore isolated from S. coelicolor.
Fig 3.
1H NMR spectrum (a) displays the characteristics proton signals and 13C NMR spectrum (b) reveals distinct carbon resonance of the siderophore produced by S. coelicolor.
Fig 4.
GC-MS analysis of the siderophore produced by S. coelicolor.
Prominent peaks with their corresponding mass spectra supporting its structural characterization.
Table 3.
Determination of siderophore-metal complexation.
Fig 5.
The siderophore produced by S. coelicolor exhibits photoreactive properties, showing a slight shift in its λ max upon exposure to sunlight.
Fig 6.
Dose–response curve depicting the concentration-dependent antimicrobial (a&b) and antifungal (c & d) activity of the cell free supernatant (a & c) and the siderophore (b & d) produced by S. coelicolor illustrating the comparative efficacy of the crude extract and the siderophore.
Fig 7.
Antioxidant potential of the siderophore and cell free supernantant of S. coelicolor along with the standard (Gallic acid) following DPPH assay.
Table 4.
Biofilm inhibition potential of the purified siderophore and cell free supernatant of S. coelicolor.
Fig 8.
Inhibition of violacein pigment production in Chromobacterium violaceum by (a) Desferrioxamine (b) Cell Free Supernatant (c) Purified siderophore demonstrating their quorum quenching activity.
Fig 9.
Microphotographs of MCF7 cancer cells.
(a) untreated cells exhibiting normal morphology and high cell density; (b) cells exposed to the IC₅₀ concentration of purified siderophore; and (c) cells treated with the commercially available siderophore, desferrioxamine. Both treatments show decreased cell density and distinct morphological alterations characteristic of cell death.
Fig 10.
Three-dimensional response surface plot showing the effect of different variables on siderophore production in S. coelicolor.
Table 5.
Analysis of variance (ANOVA) for the polynomial model of siderophore production.