Fig 1.
Experimental flow chart of this study.
Fig 2.
Representation of the experimental operation: (a) The pulpitis model was constructed by access opening.
(b) The pulpotomy was performed using Er:YAG laser. (c) After the pulpotomy under the microscope. (d) Maxillary bone specimen.
Table 1.
Scores used during the histological examination: inflammatory cell response, tissue disorganization, and reactionary dentin formation.
Fig 3.
(a) HE staining (×40) and local magnification (×100) histological images represent dental pulp tissue after 24 hours of access opening.
(b) HE staining histological images represent the pulp tissue of mechanical group and laser group at 3d, 7d, 14d and 28d after pulpotomy(×100). Scale bar = 200 μm. ✱ pulp access cavity (The dark brown substance at the pulp access cavity was identified as residual pulp capping material); D dentin; # Inflammatory cells; ※ Capillaries; ◎ Hyperplastic calcified tissue.
Table 2.
Total histological scores at different time points after pulpotomy in the two groups (M (Q1-Q3)).
Table 3.
MOD values of IL-1β positive expression at different time points after pulpotomy in the two groups (x̄ ± s).
Fig 4.
(a) IHC staining images represent both groups of IL-1β levels at 3d, 7d, 14d and 28d after pulpotomy (×200).
(b) IHC staining images represent both groups of Par3 levels at 3d, 7d, 14d and 28d after pulpotomy (×200).Scale bar = 100μm.
Table 4.
MOD values of Par3 positive expression at different time points after pulpotomy in the two groups (x̄ ± s).